Ccdc63 (NM_001289811) Mouse Untagged Clone

CAT#: MC227961

Ccdc63 (untagged) - Mouse coiled-coil domain containing 63 (Ccdc63), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc63
• Synonyms: 4921511C16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227961 representing NM_001289811
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTAAGCCTCGTGAAGTCTCCGAAGAACCTGGACATCAACCAGAAAAACTTCATGGAACTACGCT
  TCCTGCTGCAGACCAAGGGGGACTACGAGGCGCTCATCTCCTCCATGAAGGTGCTGCTGGGAGAACTGGA
  TGATAAGATCGTTCAGATGGAGAGAAAGATCACGAACCAGAGACAGCTCTTCTTGAGGACACAAGAGGCC
  AATAATCCCAAGAGACTGCAGAGACAGATCCATGTTCTGGAGTCCCGACTGAACCTGGTCACTGTCCACT
  TTGACACGATGCTGACCTCTAACTCCCAGCTCCGGAAGGAGATTGAGGATCTCCTCTTTGAGAAGGCCGC
  CTACGACCACGTCTACCAGCAGCTTCAGCGGCGTCTGCAGACACAGAAGAAGACCATGAACGTGGCCATT
  GAGCAGTCTGCCCAGGCCTATGAGCAGAGAGTGGAGGCCATGGCTCGGATGGCTGCCATGAAGGACCGCC
  AGCAGAAAGACATCTCTCAGTACAACCTGGAGATCCGGGAACTGGAACGTCTATATGATCACGAGACGAA
  ACTCAAGTCCTTCCTGCTGGCCAAGCTTAACGACCGCTCTGAATTTGAGGACCAGGCCAAGAAGCAGGAA
  GATGTCAAATCCAAGAAGCTTGGGAAGAAGGGGAAGGGCGAGAGTTTCGCGAGCTATGAGGTGGCGCACC
  TGCGGCTGCTGAAGCTGGCGGAGAACGGGGACCTGAACCAGCTCACCGAGGATTTCCTGGCCAAGGAGGA
  GAAGAATTTTGCTCGTTTCACTTATGTCACCGAGCTCAACAATGACATGGAGACCATGCACAAGAAGACC
  CAGCGGATCCAGGATGACATCATCAACTTGCGGTCACAGCAGCAGACCTCCCATGAGGGCACCCGGAGCA
  TCCTGAAACAGATGGAGGAGAAACTGAGAAAGACCACGCAGGAAGCGGACATTTATGAGACCAAATACAA
  GGAGATGAGCAAGACCCTGGAGTATCTCAAGAACTCGGTGGAGAAGATGTTTAAGAAGATCAACTGCGAT
  GCCACTGAGATCCTGGGCAAGCTGGGCGAGTCCGGGAAGGTCACGGACATCAATCTGCAGCAGTATTTTG
  CTATCATAGAAAAGAAGACAAATGACCTGCTGCTTCTAGAGTCCTTCCGGCGCCTGCAGGAGGCAGAGGG
  GCCCGATGTCGATGTCCCACAACCCTTTGTCAACCCCTTCTGGGGCGGCTCTGCCCTCCTCAAGCCCCCA
  GAGCCCATAAGGGTTGTGCTGCCTGTGTTTGGGGCAGACTCCTTCAGTGACAAACTTGAAGAGGTGGACT
  CTCCTCTGGACCACAGTACCCTCCAGCAGATGGTCTTGGAGAACTTCCTTCAGAGGGAACGCACAAAAGA
  ACTACAGGACACCATGTCAGAGAAGGGGGACGAGATAAGGCTCAAGAAGAAGGTGATAGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289811
• Insert Size: 1464 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289811.1, NP_001276740.1
• RefSeq Size: 2145 bp
• RefSeq ORF: 1464 bp
• Locus ID: 330188
• UniProt ID: Q8CDV6
• Cytogenetics: 5 F
• Gene Summary: Plays a role in spermiogenesis (PubMed:26501274). Involved in the elongation of flagella and the formation of sperm heads (PubMed:26501274).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, initiates translation at a downstrean start codon and uses an alternate splice site in the 3' terminal exon, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct C-terminus, compared to isoform 1.