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Nmt2 (NM_001290369) Mouse Untagged Clone

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SKU
MC227941
Nmt2 (untagged) - Mouse N-myristoyltransferase 2 (Nmt2), transcript variant 3
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $503.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nmt2
Synonyms A930001K02Rik; AI605445; AU044698; hNMT-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227941 representing NM_001290369
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGGAGACCGAACACGCCAAAGGAAGCCCTGGAGGGGATTTGGGAGCAAAAAAGAAAAAGAAGAA
ACAAAAGAGAAAAAAGGAGAAGCCAAATTCTGGGGGTACCAAGTCCGACTCTGCATCTGACTCCCAGGAG
ATTAAGATTCAGCAGTCTTCTAAACACAACGCCATCTGGCAGCAGATTTCAGCAGGAGCAGCCATGGAAT
CCCACCATCCCTATTCAGAAGCTACAAGACATCCAGAGAGCAATGGAGCTGCTGTCTGCATGCCAAGGCC
CAGCCAGGAACATTGATGAGGCCACAAAACGCAGATACCAGTTTTGGGACACACAGCCAGTGCCCAAATT
GAATGAAGTCATAACATCTCATGGTGCAATTGAACCAGACAAAGACAATATCCGCCAGGAACCATATTCT
TTGCCGCAAGGTTTTATGTGGGACACTTTAGACTTGAGTAATGCCGAAGTGCTGAAGGAGCTGTATACGT
TGCTGAATGAGAATTATGTGGAAGACGATGACAATATGTTCCGCTTTGACTATTCACCTGAGTTTCTGCT
GTGGGCTCTGCGCCCCCCAGGATGGCTTCTGCAGTGGCACTGTGGAGTCCGAGTGTCTTCAAATAAAAAG
TTAGTAGGTTTCATAAGTGCCATTCCAGCAAACATCCGAATTTATGATAGTGTGAAGAGGATGGTAGAAA
TCAACTTTCTTTGTGTCCATAAGAAACTGAGATCAAAACGGGTAGCCCCAGTGTTGATTCGAGAAATAAC
CAGAAGAGTGAACCTGGAAGGCATCTTTCAGGCTGTGTATACTGCGGGAGTAGTTCTTCCTAAGCCTGTG
GCCACTTGCAGGTATTGGCACCGATCCCTAAACCCCAGGAAACTGGTGGAAGTGAAATTTTCTCACCTGA
GTAGAAACATGACCTTACAGAGAACGATGAAGCTTTACAGACTTCCAGATGTTACAAAGACTTCAGGTTT
GAGACCAATGGAACCAAAAGACATCAAAGCAGTCCGAGAGCTAATCAACATCTACTTGAAGCAGTTTCAT
CTAGCTCCAGTGATGGATGACGCAGAAGTGGCCCACTGGTTCCTGCCCAGGGAGCACATCATTGACACGT
TTGTAGTGGAGAACCCCAGTGGGAAGCTGACTGACTTCCTGAGCTTCTACACTCTCCCCTCCACGGTTAT
GCACCACCCAGCTCACAAAAGCCTCAAGGCTGCCTATTCCTTCTACAACATTCACACAGAGACACCCCTG
CTGGACCTCATGAATGATGCGCTCATTATAGCCAAATTGAAAGGATTTGATGTGTTCAATGCACTAGATT
TGATGGAAAATAAGACCTTCTTGGAAAAACTAAAGTTTGGTATAGGAGATGGCAACTTACAGTATTATTT
GTACAACTGGAGGTGTCCAGGGACAGACTCTGAAAAGGTTGGACTTGTTCTACAGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001290369
Insert Size 1458 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001290369.1, NP_001277298.1
RefSeq Size 4411 bp
RefSeq ORF 1458 bp
Locus ID 18108
Cytogenetics 2 A1
Summary Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) lacks an alternate exon that results in translation initiation at an alternate start codon and a frameshifted 5' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001290369" in other vectors (1)
SKU Description Size Price
MR230152 Nmt2 (myc-DDK-tagged) - Mouse N-myristoyltransferase 2 (Nmt2), transcript variant 3 10 ug
$289.00 $457.00

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