Usp3 (NM_001302116) Mouse Untagged Clone

CAT#: MC227931

Usp3 (untagged) - Mouse ubiquitin specific peptidase 3 (Usp3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp3
• Synonyms: AA409661; BC017156
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227931 representing NM_001302116
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTGTCCGCACCTCAGCTCCAGCGTCTGCATCGCCCCGGACTCAGCCAAGTTCCCCAACGGCTCCC
  CGTCGTCCTGGTGCTGCAGCGTGTGCAGGTCCAACAAAAGCCCCTGGGTCTGTTTGACTTGTTCAAGTGT
  CCACTGTGGAAGGTATGTGAATGGCCATGCAAAAAAACATTATGAAGATGCACAGATACCCTTACTCAAC
  CATAAGAGATCAGAAAAGCAGGAGAAAGCTCAGCATACAGTGTGTATGGATTGCAGTAGCTACAGCACAT
  ACTGTTATCGCTGTGACGATTTTGTGGTCAACGACACCAAGCTGGGCCTGGTACAGAAAGTGAGAGAACA
  CTTACAGAACTTGGAAAACTCAGCCTTTACAGCGGACAGGCACAGAAAAAGAAAATTATTGGAAAACTCA
  TCACTAAACAGCAAGTTATTAAAAGTAAATGGAAGTACGACTGCCATTTGTGCCACAGGCCTTCGAAATT
  TGGGCAACACTTGTTTCATGAATGCTATCCTTCAGTCACTCAGGTCTTTAGTAGAAGAGTTTAGAAAGAC
  ACTGTGTGCTTTATGGCAAGGCAGCCAGACTGCATTTAGTCCTGAGTCCTTGTTTTATGTTGTCTGGAAG
  ATCATGCCTAACTTTAGGGGTTATCAGCAGCAGGATGCCCATGAATTCATGCGCTACCTTTTGGATCATC
  TACACTTAGAACTTCAGGGTGGCTTCAATGGTGTTTCCCGCTCAGCAATTCTACAGGAGAATTCTACACT
  GTCTGCAAGTAACAAATGCTGCATAAATGGAGCATCAACTGTTGTCACAGCAATATTTGGAGGAATTCTC
  CAGAACGAGGTCAACTGCCTCATATGTGGGACAGAGTCGAGGAAGTTTGATCCATTCCTAGATCTCTCCT
  TAGATATTCCAAGTCAGTTCAGAAGCAAGCGCTCTAAGAATCAGGAAAATGGGCCAGTTTGCTCCTTAAG
  AGATTGCCTTCGCAGCTTCACTGACCTAGAGGAACTTGATGAGACAGAGTTGTATATGTGCCATAAGTGC
  AAAAAGAAACAAAAGTCCACAAAGAAGTTTTGGATTCAAAAACTTCCCAAGGCACTGTGCTTGCACCTGA
  AAAGGTTTCACTGGACAGCATATTTAAGAAACAAAGTCGATACATACGTTCAATTCCCACTGAGAGGCCT
  GGACATGAAGTGCTACCTACTAGAGCCGGAGAACAGTGGTCCAGACAGTTGCCTGTATGACCTCGCTGCT
  GTGGTTGTACACCATGGCTCTGGGGTTGGTTCTGGACATTACACAGCATATGCAGTTCACGAAGGCCGCT
  GGTTCCATTTCAATGACAGCACTGTGACAGTGACTGATGAGGAGACCGTTGGGAAGGCAAAGGCCTACAT
  TCTGTTTTATGTGGAGCGTCAGGCCAGAGCTGGAGCAGAAAAACTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302116
• Insert Size: 1449 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302116.1, NP_001289045.1
• RefSeq Size: 5480 bp
• RefSeq ORF: 1449 bp
• Locus ID: 235441
• Cytogenetics: 9 C
• Gene Summary: The protein encoded by this gene is a chromatin-associated histone 2A and 2B deubiquitinating enzyme that negatively regulates the DNA damage response. Mice deficient for this enzyme have reduced hematopoietic stem cell reserves, demonstrating a requirement in hematopoietic stem cell homeostasis. In addition, knock down of protein levels results in spontaneous tumor development and shortened lifespan, consistent with a function in preserving chromosomal integrity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region. This results in a shorter protein (isoform 2), compared to isoform 1.