Pxylp1 (NM_001289646) Mouse Untagged Clone

CAT#: MC227913

Pxylp1 (untagged) - Mouse 2-phosphoxylose phosphatase 1 (Pxylp1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pxylp1
• Synonyms: 9430094M07Rik; Acpl2; BB177120; C130099A20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227913 representing NM_001289646
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCACCGTAATCGCTTCTTGGTGCTGCTGGCCCTGGCTGGGCTCTTGGCTTTCCTGAGCCTCAGCC
  TGCAGTTCTTCCACTTGATCCCAGTGTCTGCCACAAAGAATGGAGGAAGCAGCAAGAGTCGAAAGAGGAT
  CATGCCTGACCCAGTGACAGAGCCTCCCACCGTAGACCCGGTTTATGAAGCTCTTCTGTACTGCAACATT
  CCAAGCGTAGCGGAGCACAGCATGGAAGGTCATGCCCCGCATCATTATAAGCTGGTCTCCGTTCATGTGT
  TCATTCGCCATGGGGACAGGTACCCACTGTATGCCATTCCCAAAACAAAGCGGCCAGAAATCGACTGCAC
  TCTAGTGGCTAGCAGGAAGCCGTATCACCCTAAACTAGAAGCTTTCATTAGTCACATGTTGAAAGGATCC
  GGAGCCTCTTTTGAAAGCCCCTTAAATTCCCTGCCTCTCTATCCTAACCATCCTCTGTGTGAGACGGGAG
  AGCTCACGCAGACAGGAGTCGTGCAGCATCTGCTGAATGGCCAGCTGCTGAGGGACATCTATCTGAGGAA
  ACACAAACTTCTGCCCAACAACTGGTCCTCAGACCAGCTTTACCTGGAGAGCACGGGGAAGAGCCGCACC
  CTGCAGAGCGGGCTAGCCCTGCTCTACGGCTTCCTCCCAGAGTTTGACTGGAAGAAGGTTTATTTCAAGC
  ACCAGCCAAGTGCCCTGTTCTGCTCTGGAAGCTGCTACTGCCCGCTGAGAAACCAGTATCTGGAGAAGGA
  ACAGCGACGCCAGTACCTGCTACGTTTGAAGAACAGCGACCTAGAGAGGACCTACGGGGAGATGGCCAAG
  ATTGTGGACATCCCCACGAAGCAGCTCCGCGCGGCCAACCCCATCGATTCCATGCTCTGCCACTTCTGCC
  ACAATGTCAGCTTCCCCTGCAGCAGAAGCGGCTGCCTTGGCATGGAGCACTTCAAGGTGATCAAGACGCA
  CCAGATAGAGGATGAGAGGGAGCGCCACGAGAAGCTCCTGTACTTTGGGTACTCCCTCCTCGGGGCCCAC
  CCCATCCTAAATCAGACGGTCAACCGGATGCAGCGCGCTGCCTCGGGCTGGAGGGATGAGCTGTTCACCC
  TCTACTCTGCTCATGACGTCACCCTGTCACCCATCCTCAGTGCCTTGGGTCTTTTGGAAGCCAGGTTCCC
  GAGGTTTGCCGCCAGGCTGGTTTTTGAGCTCTGGCAAGACCGTCAAAAGCCCAGTGAACATTCGGTCCGA
  ATTCTTTATAACGGGGCTGATGTTACCTTCCACACCTCCTTCTGCCATGACTTCCACAAGCGCTCTCCCA
  AGCCTATGTGTCCTCTTGAGAACTTGGTCCGCTTTGTCAAACGGGATATGTTTGTGGCCCTGGATGGCAG
  TAGTACTAACTATTACGATGCCTGTCATGGGGAAGGGGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289646
• Insert Size: 1443 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289646.1, NP_001276575.1
• RefSeq Size: 3052 bp
• RefSeq ORF: 1443 bp
• Locus ID: 235534
• UniProt ID: Q8BHA9
• Cytogenetics: 9 E3.3
• Gene Summary: Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.