Aldh3b3 (NM_028545) Mouse Untagged Clone

CAT#: MC227906

1700055N04Rik (untagged) - Mouse RIKEN cDNA 1700055N04 gene (1700055N04Rik)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aldh3b3
• Synonyms: 1700055N04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227906 representing NM_028545
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCAAAGGCAAACACCCACGAGCAGATCAGGGGACGGACCCTTTTGAAGAAAAGCTACAGAGGC
  TGAAGGAGGCCTTCAACACAGGGAAGACAAAGACGGCTAAGTTCAGGGCTGAACAACTGCAGAGCCTGGG
  CCGCTTCCTGCAGGACAATAGCAAACAGCTTCATGACGCCCTGGATGGAGACCTGGGCAAGTCAGGCTTC
  GAGTCAGACATGAGTGAGATCATTCTGTGCGAGAATGAGGTGGACCTGGCCCTCAAGAACCTGCAGACCT
  GGATGAAGGATGAGCCTGTGTCCACCAACTTGCTCACCAAGCTGAGCACAGCTTTCATCCGGAAAGAGCC
  CTTTGGCCTGGTGCTTATCATTGCACCCTGGAATTATCCTGTGAACTTGATGATCATACCTTTAGTGGGG
  GCCATTGCTGCAGGAAACTGTGTGGTGCTGAAGCCATCAGAAATTAGCAAGAACACAGAGAAGGTGCTGG
  CAGAGCTGCTACCCCAGTATCTGGACCAGAGCTGCTTTGCTGTGATGTTGGGAGGACCCGAGGAGACCGG
  GCAGCTGCTGGAACACAAATTTGACTATATCTTCTTCACAGGAAGTCCTCGGGTAGGCAAGATCGTCATG
  ACTGCTGCTGCCAAACACCTGACACCCATCACCCTGGAGCTGGGGGGTAAGAACCCCTGCTATGTGGATG
  ACAACTGCGACCCCCAGACAGTGGCCAACCGTGTGGCCTGGTTCCGCTATTTCAATGCCGGCCAGACCTG
  TGTGGCCCCCGATTATATCCTGTGTAGCCAGGAGATGCAGGAGCAGTTGGTGCCTGCCCTGCAGAATGCC
  ATCACGCGTTTCTATGGAGATAACCCACAGACCTCCCCCAACCTGGGCAGAATCATCAACCAGAAGCATT
  TCAAGAGGCTCCAGGGATTGCTGGGCTGTGGCCGTGTGGCCATCGGTGGCCAGAGTGATGAGGGAGAGCG
  CTACATTGCCCCCACAGTGTTAGTGGACGTGCAGGAGACAGAGCCCGTGATGCAGGAGGAGATCTTTGGG
  CCCATCCTGCCCCTGGTGACTGTGAGAAGCCTGGATGAAGCCATCGAGTTCATGAACCAGCGGGAAAAGC
  CGCTAGCGCTTTATGCCTATTCCAACAACGCAGAGGTCATCAAACAGGTGTTGGCCCGGACCAGCAGCGG
  GGGCTTCTGTGGGAACGATGGCTTCATGTACATGACCCTGTCCAGCCTTCCTTTTGGAGGAGTGGGATCG
  AGTGGGATGGGCAGGTACCACGGCAAGTTCTCCTTTGACACCTTCTCCAACCAGCGTGCCTGTCTGCTGA
  GCTGCCCTGGGATGGAGAAGCTTAATGGCCTCCGTTACCCGCCCTATAGTCCCCGTAGGCAGCAGCTGCT
  AAGATGGGCTATCGGCTCTGAGAGCTGCACCCTTCTGTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_028545
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028545.2, NP_082821.2
• RefSeq Size: 1999 bp
• RefSeq ORF: 1440 bp
• Locus ID: 73458
• UniProt ID: J3QMK6
• Cytogenetics: 19 A
• Gene Summary: Oxidizes medium and long chain aldehydes into non-toxic fatty acids.[UniProtKB/Swiss-Prot Function]