Rad17 (NM_001283011) Mouse Untagged Clone

CAT#: MC227905

Rad17 (untagged) - Mouse RAD17 homolog (S. pombe) (Rad17), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rad17
• Synonyms: MmRad24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227905 representing NM_001283011
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGGAGACGATCTGACAACTGATAAGAAGATCATTCTGGTGGAAGAGTTACCTAACCAGTTTTATC
  GAGACCCTAACGCTTTACATGAGATTCTAAGAAAGCATGTGCAGATTGGCCGGTGTCCTCTTGTATTTAT
  AGTCTCTGACAGTGTCAGTGGAGACAATAATCAGCGGCTACTCTTCCCCAGAAACATTCAAGAAGAGTGT
  TCTGTTTCAAACATTAGTTTCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATTGTGA
  CTATAGAAGCTAGTAAGAATGGAGAAAAAATTATTGTTCCTAATAAAACTTCTCTGGAGTTGCTCTGTCA
  AGGATGTTCTGGTGATATCAGAAGTGCAATAAACAGCCTGCAGTTCTCGTCTTCAAAAGGAGAGAACAGT
  TCGTGGTCAAAGAAGAAAAGAATGTCATTGAAATCGGATGCTGCCATATCAAAATCAAAACAAAAAAAGA
  AACATAATAGTACTTTGGAAAATCAAGAGATTCAAGCTATTGGTGGAAAAGATGTTTCTCTTTTTCTCTT
  CAGAGCTTTGGGGAAGATTCTGTACTGTAAAAGAGCGCCTTTAACAGAGTTAGACTCACCTCGCCTGCCT
  GCTCACTTGTCAGAGCACGACCGGGACACTTTACTTGTTCAGCCTGAGGAAATAGTAGAAATGTCACACA
  TGCCAGGAGACTTTTTTAATTTATACCTTCACCAAAACTACATAGATTTCTTCGCTGAAGTAGATGATCT
  TGTGCCAGCCAGTGAGTTTCTGAGTTTTGCAGATATCCTCGGTGGTGATTGGAATACACGCTCGTTACTC
  AGGGAATATAGTACATCCGTAGCTACAAGAGGTGTGATGCATTCCAACAAAGCCCGGGGGTTCGCTCACT
  GCCAAGGAGGATCAAGCTTTCGACCCTTACATAAGCCCCAGTGGTTTCTAATACAGAAAAAGTATCGGGA
  AAATTGCCTGGCAGCAAAAGCACTCTTTGTTGACTTCTGCCTACCAGCTTTGTGTCTCCAGACTCAGCTC
  TTGCCATATCTTGCTCTTCTGACCATTCCAATGCGAAATAAAGCTCAGATTTCTTTTATCCAAGATGTTG
  GAAGACTTCCTTTGAAGCGAAGCTTTGGAAGGTTGAAAATGGAAGCCCTGACTGACCGAGAGCTGGGGCT
  GATAGACCCTGACAGTGGGGATGAGTCCCCGCACAGCGGAGGGCAGCCGGCTCAGGAAGCTCCCGGTGAA
  CCCGCGCAGGCTGCTCAGAATGCCGACCCAGAGACCTGGTCCCTTCCGCTGAGTCAGAACAGCGGGAGCG
  ATCTGCCTGCCAGCCAGCCTCAGCCCTTTTCCTCCAAAGTGGACATGGAAGAAGAGGAGGAGGAAGAGGA
  AGACATAATAATAGAGGACTATGACAGCGAGGAGACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001283011
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001283011.1, NP_001269940.1
• RefSeq Size: 2940 bp
• RefSeq ORF: 1440 bp
• Locus ID: 19356
• UniProt ID: Q6NXW6
• Cytogenetics: 13 D1
• Gene Summary: Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination (By similarity). Essential for embryonic development. May be involved in homologous recombination.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate exon in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.