Trpc7 (NM_001302373) Mouse Untagged Clone

CAT#: MC227893

Trpc7 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpc7
• Synonyms: TR; TRP7; Trrp8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227893 representing NM_001302373
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTTGTGGCCCATGCAGTGTCTTTTACCATCTTCTTGGGACTGCTAGTTGTGAACGCCTCCGACC
  GGTTTGAGGGCGTTAAGACTCTGCCCAATGAGACCTTCACAGACTACCCCAAACAGATCTTCAGAGTGAA
  AACCACACAGTTCTCCTGGACGGAGATGCTCATCATGAAGTGGGTCTTAGGAATGATCTGGTCCGAATGC
  AAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGTGCTACACTTGTGGAACCTTCTGGATTTTGGCATGC
  TGTCCATCTTCGTGGCCTCCTTCACTGCGAGGTTCATGGCTTTCCTCAAGGCCAGCGAGGCCCAGCTGTA
  CGTGGACCAGTACGTGCAGGATGTAACGCTGCACAACGTCTCACTTCCACCGGAAGTGGCCTACTTCACC
  TACGCCAGGGATAAGTGGTGGCCTTCAGACCCCCAGATCATCTCGGAAGGGCTGTACGCCATTGCGGTTG
  TCCTCAGCTTCTCCCGGATCGCCTACATCCTGCCAGCCAATGAGAGCTTCGGACCTTTGCAGATCTCCCT
  GGGAAGAACTGTGAAAGACATCTTCAAGTTCATGGTCATTTTTATCATGGTATTTGTGGCCTTTATGATC
  GGAATGTTCAACCTGTACTCCTACTACCGAGGTGCAAAGTACAACCCAGCGTTTACCACAGTTGAAGAAA
  GCTTTAAAACCTTGTTTTGGTCCATATTTGGCTTGTCTGAGGTCATCTCGGTGGTGCTGAAATACGACCA
  CAAGTTCATCGAGAATATTGGCTACGTGCTGTATGGGGTTTATAATGTCACCATGGTGGTTGTACTACTC
  AACATGCTGATAGCCATGATCAACAACTCCTATCAGGAAATCGAGGAAGACGCGGACGTGGAGTGGAAAT
  TTGCTCGAGCGAAGCTCTGGCTTTCTTACTTTGATGAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGT
  GCCGAGCCCTAAATCCTTTTATTATCTCATAATGAGAATCAAGATGTGCCTCATAGAGCTCTGCCAATCT
  AAGGCCAAACGCTGTGAAAACGACCTGGAAATGGGCATGCTGAACTCCAAGTTCAGGAAGACTCGCTACC
  AGGCTGGCATGAGGAATTCTGAAAACCTGACAGCCAATAGCACCTTCAGCAAGCCCACCAGATACCAGAA
  GATCATGAAGCGGCTCATAAAGAGATACGTGCTGAAGGCCCAGGTGGACAGAGAGAACGATGAAGTCAAT
  GAAGGTGAACTCAAGGAGATCAAACAAGACATCTCAAGCCTTCGCTATGAGCTCCTGGAGGAGAAGTCTC
  AGGCTACAGGAGAGCTGGCAGACCTGATCCAGCAGCTCAGCGAGAAGTTTGGGAAGAATCTGAACAAAGA
  CCACCTGCGGGTGAACCAGGGCAAGGACATTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302373
• Insert Size: 1434 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302373.1, NP_001289302.1
• RefSeq Size: 3674 bp
• RefSeq ORF: 1434 bp
• Locus ID: 26946
• Cytogenetics: 13 B1
• Gene Summary: The protein encoded by this gene is a member of the transient receptor potential channel family of proteins, which form six-transmembrane cation-permeable channels that are calcium permeant. Knock out mice are viable but display a reduction in the gamma wave activity that precedes seizure induction in response to a muscrarinic agonist, suggesting a functional role for this protein in initiation of seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform 3, which has a shorter N-terminus compared to isoform 1.