Hadhb (NM_001289798) Mouse Untagged Clone

CAT#: MC227884

Hadhb (untagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (Hadhb), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hadhb
• Synonyms: 4930479F15Rik; Mtpb; TP-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227884 representing NM_001289798
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACCATCTTGACTTCCACTTTTAGAAACTTGTCAACTACATCAAAATGGGCTCTCAGATCTTCTA
  TAAGACCTCTGAGCTGTTCTTCCCAACTGCACTCTGCCCCAGCTGTCCAGACCAAGTCAAAGAAGACTTT
  AGCAAAACCCAATATGAAGAATATTGTGGTGGTGGAAGGGGTCCGCATTCCATTTCTGCTGTCAGGCACT
  TCGTATAAAGACCTAATGCCACATGACTTGGCTAGAGCTGCACTTTCGGGTTTGTTGCATCGGACCAATA
  TTCCAAAGGATGTTGTTGATTATATCATCTTTGGTACAGTTATTCAGGAAGTGAAAACAAGCAATGTGGC
  TAGAGAGGCTGCCCTGGGAGCTGGCTTCTCTGATAAGACTCCAGCTCACACTGTCACCATGGCTTGTATC
  TCTTCAAACCAAGCCATGACCACAGCTGTTGGTCTGATAGCTTCTGGCCAGTGTGATGTCGTCGTGGCTG
  GTGGTGTTGAGTTAATGTCTGATGTCCCTATTCGTCATTCAAGAAATATGAGGAAAATGATGCTTGATCT
  CAATAAAGCCAAGACTCTGGGCCAGCGCCTGTCCTTACTCAGTAAATTCAGATTGAATTTTCTGTCCCCT
  GAGCTCCCTGCAGTGGCTGAGTTCTCCACTAATGAGACCATGGGCCACTCTGCAGACCGACTGGCTGCTG
  CCTTTGCTGTTTCTCGAATGGAACAGGATGAATATGCACTGCGTTCTCATAGTCTGGCCAAGAAGGCACA
  GGATGAAGGACACCTTTCTGATATTGTACCCTTCAAAGTACCAGGAAAGGACACAGTTACCAAAGATAAT
  GGGATCCGTCCTTCCTCACTGGAGCAAATGGCCAAACTAAAACCTGCGTTCATCAAACCCTATGGCACAG
  TGACAGCTGCAAATTCTTCTTTCCTGACTGATGGCGCTTCTGCGATGCTAATCATGTCAGAGGACAGAGC
  TCTGGCCATGGGTTATAAACCAAAGGCATATTTGAGGGATTTTATATATGTGTCCCAGGATCCGAAAGAT
  CAGCTTTTACTCGGACCAACATATGCTACTCCAAAAGTTTTAGAAAAGGCAGGGTTAACCATGAATGATA
  TCGATGCTTTTGAATTTCATGAAGCCTTCTCAGGCCAGATTTTAGCTAACTTTAAAGCCATGGATTCTGA
  TTGGTTTGCACAAAACTACATGGGTAGGAAAACCAAGGTTGGATCACCTCCTCTGGAGAAGTTTAATATC
  TGGGGCGGATCACTGTCTCTGGGGCACCCTTTTGGAGCCACTGGCTGTCGGCTGGTCATGGCAGCTGCCA
  ACAGACTGAGGAAGGATGGAGGCCAGTATGCTTTAGTGGCTGCCTGTGCAGCTGGAGGACAGGGTCATGC
  TATGATTGTGGAAGCTTACCCCAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289798
• Insert Size: 1428 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289798.1, NP_001276727.1
• RefSeq Size: 2092 bp
• RefSeq ORF: 1428 bp
• Locus ID: 231086
• UniProt ID: Q99JY0
• Cytogenetics: 5 B1
• Gene Summary: Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.