Hadhb (NM_001289798) Mouse Untagged Clone

SKU
MC227884
Hadhb (untagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (Hadhb), transcript variant 1
  $503.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Hadhb
Synonyms 4930479F15Rik; Mtpb; TP-beta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227884 representing NM_001289798
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTACCATCTTGACTTCCACTTTTAGAAACTTGTCAACTACATCAAAATGGGCTCTCAGATCTTCTA
TAAGACCTCTGAGCTGTTCTTCCCAACTGCACTCTGCCCCAGCTGTCCAGACCAAGTCAAAGAAGACTTT
AGCAAAACCCAATATGAAGAATATTGTGGTGGTGGAAGGGGTCCGCATTCCATTTCTGCTGTCAGGCACT
TCGTATAAAGACCTAATGCCACATGACTTGGCTAGAGCTGCACTTTCGGGTTTGTTGCATCGGACCAATA
TTCCAAAGGATGTTGTTGATTATATCATCTTTGGTACAGTTATTCAGGAAGTGAAAACAAGCAATGTGGC
TAGAGAGGCTGCCCTGGGAGCTGGCTTCTCTGATAAGACTCCAGCTCACACTGTCACCATGGCTTGTATC
TCTTCAAACCAAGCCATGACCACAGCTGTTGGTCTGATAGCTTCTGGCCAGTGTGATGTCGTCGTGGCTG
GTGGTGTTGAGTTAATGTCTGATGTCCCTATTCGTCATTCAAGAAATATGAGGAAAATGATGCTTGATCT
CAATAAAGCCAAGACTCTGGGCCAGCGCCTGTCCTTACTCAGTAAATTCAGATTGAATTTTCTGTCCCCT
GAGCTCCCTGCAGTGGCTGAGTTCTCCACTAATGAGACCATGGGCCACTCTGCAGACCGACTGGCTGCTG
CCTTTGCTGTTTCTCGAATGGAACAGGATGAATATGCACTGCGTTCTCATAGTCTGGCCAAGAAGGCACA
GGATGAAGGACACCTTTCTGATATTGTACCCTTCAAAGTACCAGGAAAGGACACAGTTACCAAAGATAAT
GGGATCCGTCCTTCCTCACTGGAGCAAATGGCCAAACTAAAACCTGCGTTCATCAAACCCTATGGCACAG
TGACAGCTGCAAATTCTTCTTTCCTGACTGATGGCGCTTCTGCGATGCTAATCATGTCAGAGGACAGAGC
TCTGGCCATGGGTTATAAACCAAAGGCATATTTGAGGGATTTTATATATGTGTCCCAGGATCCGAAAGAT
CAGCTTTTACTCGGACCAACATATGCTACTCCAAAAGTTTTAGAAAAGGCAGGGTTAACCATGAATGATA
TCGATGCTTTTGAATTTCATGAAGCCTTCTCAGGCCAGATTTTAGCTAACTTTAAAGCCATGGATTCTGA
TTGGTTTGCACAAAACTACATGGGTAGGAAAACCAAGGTTGGATCACCTCCTCTGGAGAAGTTTAATATC
TGGGGCGGATCACTGTCTCTGGGGCACCCTTTTGGAGCCACTGGCTGTCGGCTGGTCATGGCAGCTGCCA
ACAGACTGAGGAAGGATGGAGGCCAGTATGCTTTAGTGGCTGCCTGTGCAGCTGGAGGACAGGGTCATGC
TATGATTGTGGAAGCTTACCCCAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001289798
Insert Size 1428 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001289798.1, NP_001276727.1
RefSeq Size 2092 bp
RefSeq ORF 1428 bp
Locus ID 231086
UniProt ID Q99JY0
Cytogenetics 5 B1
Summary Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
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Documents
Resources
"NM_001289798" in other vectors (1)
SKU Description Size Price
MR230095 Hadhb (myc-DDK-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (Hadhb), transcript variant 1 10 ug
$289.00 $457.00

file-alt Citations

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