Bpifb1 (NM_153418) Mouse Untagged Clone

CAT#: MC227875

Bpifb1 (untagged) - Mouse BPI fold containing family B, member 1 (Bpifb1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bpifb1
• Synonyms: Lplunc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227875 representing NM_153418
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCCCGTGGATTATCACCCTCCTCTGTGGTTTGCTGGGAGCCACACTGGTCCAAGCTAACGTCT
  ATCCCCCTGCAGTGCTCAACCTTGGCCCAGAAGTCATCCAGAAACACCTGACCCAGGCACTGAAGGACCA
  TGATGCCACTGCCATCCTCCAGGAGTTGCCACTGCTCAGAGCCATGCAAGATAAGTCTGGCAGTATCCCC
  ATACTGGACAGCTTCGTGCACACCGTTCTGAGATACATCATATGGATGAAGGTCACCTCTGCTAACATCC
  TCCAGCTGGATGTGCAGCCTTCAACTTATGACCAGGAGCTGGTGGTCAGAATCCCCCTGGACATGGTGGC
  TGGACTAAACACACCACTGATCAAGACCATAGTGGAGTTCCAAATGAGCACCGAGGTCCAAGCCCTCATC
  CGGGTGGAGAGGAGCAAGAGCGGCCCCGCCCACCTGAATCTCAGCGACTGCTCCAGCAATGAGAGCACCC
  TGCGCCTCAGCCTGCTTCACAAGCTCTCCTTCGTGGTCAACTCCTTGGCAAAGAATGTTATGAATCTCCT
  GGTGCCAGCCCTGCCCCAAATAGTGAAAAACCACCTGTGCCCTGTGATCCAGCAGGCCTTTGATGACATG
  TACGAAGACTTCCTGAGACTGACAACAGCACCCATTGCCCTCAGTCCTGGAGCCCTGGAGTTTGGCCTTC
  TGTCTCCTGCTATCCAGGACAGTAATATCCTGTTGAACCTGAAGGCCAAGCTGCTGGACTCACAGGCGAG
  GGTAACCAACTGGTTCAACAACTCTGCGACTTCTCTGATGGAGACCACCCCAGACAGGGCCCCCTTCAGC
  CTGACCGTGAGGCAGGACCTGGTGAATGCCATTGTGACCACCCTGGTCCCCAAGGAGGAGCTTGTAATCC
  TGCTCAGATTCGTGATTCCTGATGTGGCCCGCCAGTTACAGATGGACATCAAGGAAATCAATGCAGAGGC
  AGCCAACAAGCTGGGGCCCACCCAGATGTTGAAGATCTTCACCCACAGCACCCCCCACATTGTGCTGAAC
  GAGGGCAGTGCCAGGGCAGCCCAAAGTGTCGTGCTGGAAGTGTTCCCTACCAACACTGATGTCCGGCCCT
  TCTTCTCTCTCGGCATTGAGGCCAGTTATGAAGCTCAGTTCTTCACAGAAGACAACCGGCTTATGCTCAA
  CTTCAATAACGTCAGTATTGAGCGCATCAAGCTGATGATCTCAGATATCAAACTATTCGATCCTGAAGTC
  CTGAAGGACACCCTGACCAAGATCCTTGAATACACACTGCTGCCCAATGAGAATGGCAAACTGAGAACTG
  GAGTCCCCATGTCAATGTCCAAGGCCTTGGGATACGAGAAAGCCATGTGGTCTGTGAGCAAGGGTGCCCT
  CAAGCTCACTCCAGCCTCCTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153418
• Insert Size: 1425 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153418.2, NP_700467.2
• RefSeq Size: 1653 bp
• RefSeq ORF: 1425 bp
• Locus ID: 228801
• UniProt ID: Q61114
• Cytogenetics: 2 H1
• Gene Summary: May play a role in innate immunity in mouth, nose and lungs. Binds bacterial lipopolysaccharide (LPS) and modulates the cellular responses to LPS. May be involved in formation of the left-right axis in the node of the developing embryo.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. Both variants 1 and 2 encode the same protein.