Dclre1c (NM_001302674) Mouse Untagged Clone

CAT#: MC227874

Dclre1c (untagged) - Mouse DNA cross-link repair 1C, PSO2 homolog (S. cerevisiae) (Dclre1c), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dclre1c
• Synonyms: 9930121L06Rik; A; AI661365; Art; Snm1l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227874 representing NM_001302674
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTTCGGGTGAGGAGACTTCAGACTGGCAAAAGGAGAAGCTTCCAGAATGGAGCTTCTGCACTCT
  GGAGAGTAAAAGACATCCAAAGTGTGTATTTAGACACGACTTTCTGTGACCCAAGGTTTTATCAGATCCC
  AAGTCGTGAGCAGTGTTTGAGGGGCATTTTGGAGCTGGTTCGGAGCTGGGTCACTAGGAGTCCGCACCAC
  GTCGTGTGGCTGAACTGTAAAGCAGCTTATGGCTACGAGTATTTATTCACCAACCTAAGCGAGGAGCTGG
  GAGTTCAGGTTCATGTGGACAAGCTGGACATGTTTAAAAACATGCCTGATATCCTGCACCATCTCACAAC
  GGACAGAAACACCCAGATCCACGCCTGCCGCCACCCAAAGGCAGAAGAGTGTTTTCAGTGGAATAAACTA
  CCCTGTGGTATAACTTCCCAAAACAAAACTGCACTCCACACAATCAGCATCAAGCCATCTACCATGTGGT
  TTGGAGAGAGGACCAGAAAAACCAACGTGATCGTTAGGACTGGAGAGAGCTCATACAGAGCTTGCTTCTC
  TTTTCACTCCTCCTTCAGTGAGATTAAAGATTTTTTGAGCTACATCTGCCCAGTGAATGTGTATCCAAAT
  GTCATCCCAGTTGGCCTCACTGTGGATAAGGTCATGGACGTTTTAAAGCCTCTGTGCCGGTCTCCCCAAA
  GTGTTGAGCCAAAGTACAAACCGCTTGGAAAATTGAAGAGAGCCAGAACAATCCATCTTGACTCGGAGGA
  AGACGATGATCTCTTTGATGACCCTCTACCGACACCTTTAAGGCACAAAGTTCCGTACCAGCTAACTCTT
  CAGCCTGAGCTGTTTTCAATGAAGGCACTGCCACTAGACCAGCCTGAACTGAGACAAAGCCCAGGAGGCT
  GCAAAGCAGAGAGTGTGTGGAGCCCTTCTTTGGCTAACTTCATAGACTGTGAAGAATCCAACAGCGACAG
  TGGAGAAGAGCTAGAAACCCCACCACCCTCACTGCAGGGAGGTCTGGGCCCCTCGACACTCGTCCAGCAA
  AATGCTGATCCGGATGTGGACATACCACAGTGGGAAGTCTTCTTCAAAAGAAGAGATGAAATCACAGTGG
  ACACCATGATTAGAACTCCCAGACCCAGAAAGATGAAAGGCTGTGGTCAATGGAGTCTGAAGATGCTTCT
  CCAGAACCTGGAAATACAGGAAGAGAAACATATATTTGAGAACAGGGGCTGGAAGATGGCTGGGCAGGTA
  AAGGGTAGCTGTGGTCTTTTGGAGGGCCAGAGTTCACTCCCAACATTCAAGCTAGCCACCTCCCTAGCCT
  CAAACTCCAGCTTCTGGCCTCCATGGCACCTGCATTCACATGCGCACACACAAATCTTTACCTTTAAAAA
  AAAAACCAAAACTTTACTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302674
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302674.1, NP_001289603.1
• RefSeq Size: 3896 bp
• RefSeq ORF: 1422 bp
• Locus ID: 227525
• UniProt ID: Q8K4J0
• Cytogenetics: 2 A1
• Gene Summary: This gene encodes a member of the SNM1 family of nucleases and is involved in V(D)J recombination and DNA repair. This protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Homozygous knockout mice for this gene exhibit severe combined immunodeficiency with sensitivity to ionizing radiation. Mutations in this gene in humans can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (4) differs at both the 5' and 3' ends, compared to variant 1. These differences cause translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (4) is shorter and has distinct N- and C-termini compared to isoform 1.