Tfe3 (NM_001105197) Mouse Untagged Clone

CAT#: MC227847

Tfe3 (untagged) - Mouse transcription factor E3 (Tfe3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tfe3
• Synonyms: bHLHe33; F830016E06Rik; Tcfe3; Tfe-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227847 representing NM_001105197
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCTTCTTCATCACGGGTCTTGCTGCGTCAGCAGCTTATGAGGGCCCAGGCTCAGGAACAGGAGA
  GGCGTGAGCGGCGGGAACAGGCAGCGGCCGCTCCCTTCCCCAGCCCTGCGCCCGCCTCACCAGCCATCTC
  TGTGATTGGCGTGTCTGCTGGTGGCCACACACTGAGTCGTCCACCTCCTGCTCAGGTGCCCAGGGAGGTA
  CTCAAGGTTCAGACCCACCTCGAGAACCCTACACGCTACCACCTGCAGCAAGCTCGCCGGCAGCAGGTCA
  AACAGTACTTGTCTACCACACTTGGGCCCAAGCTGGCTTCCCAGGCTCTCACCCCACCACCAGGGCCTTC
  CAGTGCCCAGCCACTTCCTGCCCCTGAAACTGCTCATGCCACCGGTCCTACAGGCAGTGCTCCTAACAGC
  CCCATGGCGCTGCTCACCATTGGGTCCAGCTCAGAAAAGGAGATTGATGATGTCATTGATGAGATCATCA
  GCCTGGAGTCCAGTTACAACGATGAGATGCTCAGCTATCTTCCAGGAGGCACTGCAGGGCTGCAGCTCCC
  CAGCACGCTGCCGGTGTCTGGAAATCTACTTGATGTGTACAGCAGTCAGGGAGTGGCTACCCCAGCTATC
  ACCGTCAGCAATTCCTGTCCAGCTGAGCTGCCTAACATCAAACGCGAGATCTCCGAAACCGAGGCAAAGG
  CCCTTTTGAAGGAGCGACAGAAGAAAGACAATCACAACCTAATTGAACGACGCAGGCGATTCAACATTAA
  CGATAGGATCAAAGAGCTGGGCACCCTCATCCCCAAGTCCAATGATCCGGAGATGCGCTGGAACAAGGGC
  ACCATCCTGAAGGCATCTGTGGATTACATCCGCAAATTACAGAAGGAACAGCAACGCTCCAAAGACCTGG
  AGAGCCGGCAGCGGTCCCTGGAACAAGCCAACCGAAGCCTGCAGCTCCGGATTCAGGAGCTAGAACTGCA
  GGCCCAGATCCATGGTCTGCCAGTCCCTCCCAACCCAGGACTGCTCTCCCTAACCACGAGTTCGGTCTCT
  GACAGCCTCAAGCCAGAACAGCTGGACATTGAGGAGGAGGGCAGGCCTAGCACAACGTTCCATGTATCGG
  GAGGACCTGCCCAGAACGCTCCTCCACAACAGCCTCCAGCACCACCTTCGGATGCCCTTCTGGACCTACA
  CTTTCCCAGCGACCACTTGGGAGACCTGGGGGACCCCTTCCACCTAGGGCTAGAGGACATTCTGATGGAG
  GAGGAGGGGATGGTGGGAGGACTGTCAGGGGGTGCCCTGTCCCCACTGCGGGCTGCTTCTGACCCCCTGC
  TTTCTTCAGTATCCCCGGCTGTTTCCAAGGCCAGCAGCCGCCGTAGCAGCTTCAGCATGGAAGAGGAGTC
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001105197
• Insert Size: 1404 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105197.1, NP_001098667.1
• RefSeq Size: 2979 bp
• RefSeq ORF: 1404 bp
• Locus ID: 209446
• UniProt ID: Q64092
• Cytogenetics: X 3.5 cM
• Gene Summary: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (PubMed:16936731). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (PubMed:16936731). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (PubMed:16936731). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer (By similarity). It also binds very well to a USF/MLTF site (By similarity). May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Variants 3, 4, and 5 encode the same isoform (c).