Slc19a2 (NM_001276455) Mouse Untagged Clone

CAT#: MC227814

Slc19a2 (untagged) - Mouse solute carrier family 19 (thiamine transporter), member 2 (Slc19a2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc19a2
• Synonyms: AV276020; AW322295; DDA1; THTR1; TRMA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227814 representing NM_001276455
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGCCAGCCAGGGTGTCCAGACGAGCGGCGGCGGCGGCGGCCAGGATGCTTCTGCGTACTGCCC
  GCGTCCCTCGCGAGTGCTGGTTCCTGCCGACCGCGCTGCTCTGCGCCTACGGCTTCTTCGCAAACCTCCG
  GCCGTCGGAGCCGTTCCTCACGCCCTACCTTCTGGGACCCGACAAGAACTTGACCGAGAGACAGGTCTAC
  AATGAAATTTATCCGGTGTGGACGTACTCTTACCTGCTGCTGCTGTTTCCCGTGTTCCTTGCCACAGACT
  ACCTCCGGTACAAGCCTGTCATCCTGCTGCAGGGACTCAGCCTGATTGTGACGTGGTTCATGCTGCTCTA
  TGCCCAGGGACTGCTGGCCATTCAGTTCTTGGAATTCTTCTACGGCATCGCCACAGCCACCGAAATCGCC
  TACTACTCCTATATCTATACTGTGGTGGACCTGGGCATGTACCAGAAAGTCACAAGCTACTGTAGAAGTG
  CCACCTTGGTGGGCTTTACAGTGGGCTCCGTCCTAGGGCAGATCCTCGTCTCCGTGGTGGGCTGGTCACT
  GTTCAGCTTGAACGTCATCTCCCTCACCTGTGTTTCTGTGGCTTTTGCTGTGGCCTGGTTTCTGCCTATG
  CCACAGAAGAGCCTCTTCTTTCACCACATTCCTTCCTCCTGTCATGGAGTGAACGGCCTCAAGGAGGAGC
  CCAAGCCAGACCGGCTGCGGGTGTTCAGAGTCCTGTGGAATGACTTCCTGATGTGTTATTCCTCCCGCCC
  TCTGCTCTGCTGGTCCGTGTGGTGGGCCCTGTCCACCTGCGGCTATTTCCAAGTGGTGAACTACGCGCAG
  GGATTGTGGGAGAAGGTGATGCCTTCTCAGAATGCTGACATCTACAATGGCGGTGTGGAGGCCGTTTCAA
  CCTTGCTGGGTGCTAGTGCTGTGTTTGCAGTTGGCTATATAAAGCTATCTTGGTCAACTTGGGGAGAAAT
  GACGTTGTTCCTGTGTTCTCTCCTGATTGCTGCTGCAGTGTATGTCATGGACACTGTGCAGAGCATCTGG
  GTGTGCTATGCATCCTATGTTGTCTTCAGAATCATCTACATGGTACTCATCACCATAGCAACTTTCCAGA
  TTGCTGCGAACCTCAGCATGGAACGTTACGCCCTTGTGTTCGGCGTGAACACCTTCATTGCCCTGGCATT
  GCAGACTCTGCTCACTCTGATTGTCGTGGATGCCAGGGGCCTTGGCTTATGTATCACCACTCAGTTCCTG
  ATTTACGCCAGTTACTTCGCAGCCATCTCTGTGGTTTTCCTGGCGAATGGCATAGTCAGTATTATAAAGA
  AATGCAGAAAGCAGGAAGATCCCAGCTCCAGCCCCCAAGCCTCCACGTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276455
• Insert Size: 1383 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276455.1, NP_001263384.1
• RefSeq Size: 3457 bp
• RefSeq ORF: 1383 bp
• Locus ID: 116914
• UniProt ID: Q9EQN9
• Cytogenetics: 1 71.56 cM
• Gene Summary: High-affinity transporter for the intake of thiamine.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (b) is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.