Glra1 (NM_001290821) Mouse Untagged Clone

CAT#: MC227795

Glra1 (untagged) - Mouse glycine receptor, alpha 1 subunit (Glra1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Glra1
• Synonyms: nmf11; oscillator; ot; spasmodic; spd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227795 representing NM_001290821
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGCTTCAATACTCTGCGATTCTACCTTTGGGAGACCATTGTATTCTTCAGCCTTGCTGCTTCCA
  AAGAGGCTGAAGCCGCCCGCTCCGCACCCAAGCCTATGTCACCCTCGGACTTCCTGGATAAGCTCATGGG
  GAGGACTTCTGGGTATGACGCCAGGATCAGGCCCAACTTTAAAGGTCCTCCTGTGAATGTAAGTTGCAAC
  ATCTTCATCAACAGTTTCGGTTCCATCGCTGAGACAACCATGGACTATAGGGTCAACATCTTCCTGAGGC
  AGCAGTGGAACGACCCCCGTCTGGCCTACAATGAATACCCTGATGACTCTCTGGACCTTGACCCATCTAT
  GTTGGATTCCATCTGGAAGCCTGACTTGTTCTTTGCCAATGAGAAGGGGGCCCACTTCCACGAAATCACC
  ACGGACAACAAACTGCTAAGAATCTCCCGGAATGGCAATGTCCTCTACAGCATCAGAATCACCCTGACGC
  TGGCCTGCCCCATGGACCTGAAGAATTTCCCGATGGATGTACAGACGTGTATCATGCAACTCGAAAGCTT
  TGGATATACCATGAACGACCTCATCTTTGAGTGGCAGGAGCAAGGAGCTGTGCAGGTGGCAGACGGACTG
  ACCCTGCCTCAGTTTATTCTGAAGGAAGAGAAAGACCTGAGATACTGCACCAAGCACTACAACACAGGTA
  AATTCACCTGCATCGAGGCCCGATTCCACCTGGAGCGGCAGATGGGCTACTACCTGATCCAGATGTACAT
  CCCCAGCCTGCTCATCGTCATCCTGTCCTGGATCTCCTTCTGGATCAACATGGATGCTGCACCAGCTCGT
  GTGGGGCTGGGCATCACCACAGTGCTCACCATGACCACACAGAGCTCTGGCTCCCGAGCCTCCCTACCCA
  AGGTGTCCTACGTGAAAGCTATTGACATTTGGATGGCTGTTTGCCTGCTCTTCGTGTTCTCTGCCCTGCT
  GGAGTACGCCGCCGTCAACTTTGTGTCTCGGCAACACAAGGAACTGCTTCGATTTAGGAGGAAGAGGCGA
  CATCACAAGAGCCCCATGCTAAATCTGTTTCAGGATGATGAGGGTGGAGAAGGCCGCTTCAACTTCTCTG
  CCTATGGGATGGGCCCAGCCTGTCTGCAGGCCAAGGATGGCATCTCTGTCAAGGGTGCCAACAACAACAA
  CACCACTAACCCGCCTCCTGCGCCATCCAAGTCCCCGGAGGAGATGCGGAAACTCTTCATCCAGAGAGCC
  AAGAAGATCGACAAGATATCTCGCATCGGTTTCCCCATGGCCTTCCTCATCTTCAACATGTTCTACTGGA
  TCATCTATAAGATCGTCCGGAGAGAGGATGTCCACAACAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290821
• Insert Size: 1374 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290821.1, NP_001277750.1
• RefSeq Size: 2413 bp
• RefSeq ORF: 1374 bp
• Locus ID: 14654
• UniProt ID: Q64018
• Cytogenetics: 11 33.12 cM
• Gene Summary: Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:16672662, PubMed:17114051, PubMed:24801766). Channel opening is also triggered by taurine and beta-alanine (By similarity). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization (By similarity). Plays an important role in the down-regulation of neuronal excitability (PubMed:9145798). Contributes to the generation of inhibitory postsynaptic currents (PubMed:16672662, PubMed:17114051, PubMed:24801766). Channel activity is potentiated by ethanol. Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol (PubMed:24801766).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).