Erg (NM_001302154) Mouse Untagged Clone

CAT#: MC227789

Erg (untagged) - Mouse avian erythroblastosis virus E-26 (v-ets) oncogene related (Erg), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erg
• Synonyms: D030036I24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227789 representing NM_001302154
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCACTATTAAGGAGGCCTTGTCAGTTGTGAGCGAGGACCAGTCACTATTTGAGTGTGCCTACG
  GAACGCCACACCTGGCTAAGACAGAGATGACCGCATCCTCTTCCAGTGACTATGGCCAGACATCCAAGAT
  GAGTCCCAGAGTCCCTCAGCAGGACTGGCTGTCTCAAGCCCCAGCCAGGGTCACCATCAAGATGGAGTGC
  AACCCTAGTCAGGTGAATGGTTCCAGGAACTCACCTGATGAGTGCAGTGTGAACAAAGGTGGGAAGATGG
  TGGGCAGCCCGGATACTGTGGGGATGAGCTACGGCAGCTACATGGAGGAGAAGCATGTGCCGCCTCCCAA
  TATGACCACAAATGAGCGCAGAGTGATCGTCCCTGCAGATCCTACTCTGTGGAGCACAGACCATGTCCGA
  CAGTGGCTGGAGTGGGCGGTGAAAGAATATGGCCTCCTCGATGTGGACGTCTTACTATTTCAGAATATCG
  ATGGGAAGGAGCTGTGCAAGATGACAAAGGATGACTTCCAGCGGCTCACGCCGAGCTACAATGCCGACAT
  TCTTCTCTCACATCTCCACTACCTCAGAGAGACTCCCCTTCCACATCTGACTTCCGATGACGTTGATAAG
  GCTTTACAAAACTCTCCACGGTTAATGCATGCCAGAAACACAGGGGGTGCAGCTTTTATTTTCCCAAATA
  CTTCAGTATATCCCGAAGCTACGCAAAGAATTACAACTAGGCCAGCTGCTCAGCCATCTCCCTCTGCAGT
  GCCCAAAACTGAAGACCAGCGTCCTCAGTTAGATCCTTACCAGATCCTGGGACCGACCAGTAGCCGCCTT
  GCTAATCCAGGTAGTGGCCAGATCCAGCTGTGGCAGTTCCTGCTCGAACTCCTGTCAGACAGCTCCAACT
  CCAACTGCATCACCTGGGAAGGCACCAACGGGGAGTTCAAGATGACAGACCCGGACGAGGTGGCTCGGCG
  CTGGGGGGAGAGGAAGAGCAAGCCCAACATGAACTATGACAAGCTCAGCCGCGCCCTCCGCTACTACTAC
  GACAAAAACATCATGACCAAGGTGCACGGGAAGCGCTACGCCTACAAGTTTGACTTCCACGGGATTGCCC
  AGGCCCTGCAGCCCCACCCTCCTGAGTCGTCCCTGTACAAGTACCCCTCCGACCTGCCATACATGGGCTC
  CTATCACGCCCACCCCCAGAAGATGAACTTTGTGTCTCCCCACCCTCCCGCTCTCCCAGTCACATCTTCC
  AGTTTCTTTGCTTCCCCGAACCCATACTGGAATTCACCGACTGGGGGCATCTACCCGAACACTAGGCTCC
  CAGCCAGCCATATGCCCTCTCACCTGGGCACCTACTACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302154
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302154.1, NP_001289083.1
• RefSeq Size: 3121 bp
• RefSeq ORF: 1371 bp
• Locus ID: 13876
• UniProt ID: P81270
• Cytogenetics: 16 56.04 cM
• Gene Summary: Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses a downstream alternate 5'-terminal exon which reflects use of an alternate promoter supported by epigenetic data, and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter and has a distinct N-terminus compared to isoform 1.