G3bp2 (NM_001080795) Mouse Untagged Clone

CAT#: MC227756

G3bp2 (untagged) - Mouse GTPase activating protein (SH3 domain) binding protein 2 (G3bp2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: G3bp2
• Synonyms: AA409541; E430034L04Rik; G3BP; mKIAA0660
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227756 representing NM_001080795
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTATGGAGAAGCCCAGTCCGCTGCTTGTGGGGCGGGAGTTTGTGAGGCAATATTATACTTTGCTGA
  ATAAAGCTCCCGAGTATTTGCACAGGTTCTATGGCAGGAATTCCTCCTATGTTCATGGTGGAGTGGATGC
  CAGTGGAAAGCCCCAGGAAGCAGTTTATGGCCAAAATGATATACACCACAAAGTGTTATCTCTGAACTTC
  AGTGAATGTCATACCAAAATTCGTCATGTGGATGCTCATGCAACCTTGAGTGATGGTGTAGTGGTACAGG
  TCATGGGCTTGCTGTCTAACAGTGGACAGCCTGAGAGGAAGTTTATGCAAACCTTTGTTCTGGCTCCAGA
  AGGATCTGTTCCAAATAAATTTTATGTTCACAATGATATGTTTCGTTATGAAGATGAAGTGTTTGGTGAT
  TCTGAACCAGAACTTGATGAAGAGTCAGAAGATGAAGTGGAAGAGGAGCAAGAAGACAGGCAGCCGTCTC
  CTGAACCTGTGCAAGAGAATGCTAACAGCGCTTACTATGACGCACACCCCGTGACTAATGGCATAGAGGA
  GCCTTTAGAAGAATCCTCTCATGAACCTGAGCCTGAGCCAGAATCTGAAACAAAGACTGAAGAGCTGAAA
  CCACAAGTGGAGGAGAAACACTTGGAAGAGCTGGAGGAGAAGTCGGCCACTCCTCCTCCTGCTGAGCCTG
  CTTCTCTGCCTCAGGAACCGCCAAAGCCGAGGGTTGATGCTAAGCCAGAAGTTCAGTCTCAGCCACCGCG
  TGTACGGGAACAGCGACCTCGAGAGCGGCCTGGTTTCCCTCCTCGAGGACCAAGGCCAGGCAGAGGAGAT
  ATGGAACAGAATGACTCTGACAACCGGAGAATAATTCGCTATCCGGACAGTCATCAGCTTTTCGTTGGTA
  ACTTGCCTCATGATATTGATGAAAATGAACTGAAAGAATTCTTTATGAGTTTTGGAAACGTTGTGGAACT
  TCGCATCAATACCAAGGGTGTTGGGGGAAAACTTCCAAATTTTGGTTTTGTGGTTTTTGATGACTCTGAA
  CCAGTTCAGAGAATCTTAATTGCAAAACCAATTATGTTTCGAGGAGAAGTACGTTTAAATGTGGAAGAGA
  AAAAAACAAGAGCTGCCAGAGAGCGAGAAACCAGAGGTGGTGGCGATGACCGCAGGGATATCAGGCGCAA
  TGATCGGGGTCCTGGTGGTCCACGTGGAATTGTGGGTGGTGGAATGATGCGTGATCGTGACGGAAGAGGG
  CCACCTCCACGAGGTGGCATGACACAGAAACTTGGTTCTGGAAGAGGAACCGGGCAAATGGAAGGTCGCT
  TCACAGGACAGCGTCGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080795
• Insert Size: 1350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080795.2, NP_001074264.1
• RefSeq Size: 4226 bp
• RefSeq ORF: 1350 bp
• Locus ID: 23881
• Cytogenetics: 5 E2
• Gene Summary: Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 3, 4, and 8 all encode the same isoform (b).