Becn2 (NM_001290692) Mouse Untagged Clone

CAT#: MC227750

Becn2 (untagged) - Mouse beclin 2 (Becn2)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Becn2
• Synonyms: Beclin-2; Gm104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227750 representing NM_001290692
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCTGCCCTCTTCCTGTGCCAGCGCTGCAAAGAACCCCTGAAACTCTTACAGCAGCAGGGGGGGC
  CCTTGGAAGTCCAGCACCACGCCAATACCCCCACAGAAATTCCAGTTTCTGCAGAGTCCCAAGTCAGGAC
  CTCCGGCAGACCCCACTCTGACGGTGGCAGGGTGTCCCAGGGAAGTGCCCTCTGCACTTTTACTCTGCTT
  ACCTCTGGCGGACCGGACTCTGAAGGTGGCACGACGTCCCAGGGAAATGCCTGCTGTACCTTTACTCTGC
  TTGGTGAATCTGCCTCCATGAGAACTATGAACACTATCCAAAATACTGTCTTAGAGACCTTCGAAATCCT
  CTCCGACCAAAAGGTGGTGGACCACCCGCTGTGTGTGGACTGTACCGACCATCTTCTGATGCAACTAGAC
  GACCAGCTCGCTCTCCTGGCATCTGACAACCAGAAGTACAAAAGTTTCCAGGACAGAGAGTTGCTGGTGA
  GTGAGGAAGAGAGGGAGGCGCTACACGCGGAGCTTTGTGCCGAACTGTCCAGTCTGGAGCAGGAGGAAGC
  AAGGCTGACCCAGGAGCTAGAGGATTTAGACGGCCATCATGCAAGGGTAGCCGCTGAACTCAGAGCAGCC
  CAGGCCGAGAGCAAGGAGCTGTATAAACAGCATGAGCAGCACAGGGTCGAGTACTCTGTTTTCAAGATGG
  AACAGCTGGAGCTGATGGACCAGCTGAGCAGTGTGGAGAACCAGCTGACGTATGCCCTGAGTCAGCAGTA
  CCGCTTAAGACAGACCAATATCTTCAACGCCACATTTACCATTTCAGATGAGGGTCCCTTGGGCGTTATC
  AACAATTTTAGGCTGGGCTGTCTCCCCGGGGTCCGGGTGGGCTGGACTGAGATCAGTTCTGCCTGGGGAC
  AGACAGTCTTACTGCTCTTCAGCTTGTCCAAGATAGCTGGACTGCAATTCCAGAGATATCAACTGGTTCC
  CTTTGGGGACCATTCCTATCTCAAGTCCTTGACTGGTGATGGCGTGCTGCCCTTATTCTCTGATGGGAGC
  CACAGCGTCTTCTTGAATAACAAGTTTGACTGTGGGATGAAGGCCTTCCTGGACTGTCTTCAGCAGTTTG
  TGGAGGAGATTGAGAGGGATGAGAGATGCCCCTGCCTGCCCTACAGGATCCATGTGAAGGAAGGGCTGAT
  GGAGGATGTCTGGGACAGTGGGGAGTGCTGCTCCATCAGGACCCATCTGAACACGGAGGAGGAGTGGTCC
  AGGGCTCTGAAATTCATGCTTTCGGACTTGAAGTTGATCCTTGCTTGGGCTTCCTTAAGGTTTTCCAGAG
  TCCAGAGGCCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290692
• Insert Size: 1344 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290692.1, NP_001277621.1
• RefSeq Size: 1789 bp
• RefSeq ORF: 1344 bp
• Locus ID: 226720
• UniProt ID: P0DM65
• Cytogenetics: 1 H4
• Gene Summary: Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.[UniProtKB/Swiss-Prot Function]