Nr1h2 (NM_001285518) Mouse Untagged Clone

CAT#: MC227728

Nr1h2 (untagged) - Mouse nuclear receptor subfamily 1, group H, member 2 (Nr1h2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nr1h2
• Synonyms: AI194859; LXR; LXRB; LXRbeta; LXRBSV; NER1; OR-1; RIP15; Unr; Unr2; UR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227728 representing NM_001285518
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCCCCACAAGTTCTCTGGACACTCCCGTGCCTGGGAATGGTTCTCCTCAGCCCAGTACCTCCG
  CCACGTCACCCACTATTAAGGAAGAGGGGCAGGAGACTGATCCTCCTCCAGGCTCTGAAGGGTCCAGCTC
  TGCCTACATCGTGGAGCCAGAGGATGAGCCTGAGCGCAAGCGGAAGAAGGGGCCGGCCCCGAAGATGCTG
  GGCCATGAGCTGTGCCGCGTGTGCGGAGACAAGGCTTCGGGCTTCCACTACAACGTGCTCAGCTGTGAAG
  GCTGCAAAGGCTTCTTCCGGCGCAGTGTGGTCCACGGTGGGGCCGGGCGCTATGCCTGTCGGGGCAGCGG
  AACCTGCCAGATGGATGCCTTCATGCGGCGCAAGTGCCAGCTCTGCCGGCTGCGCAAGTGCAAGGAGGCT
  GGCATGCGGGAGCAGTGCGTGCTCTCTGAGGAGCAGATTCGGAAGAAAAGGATTCAGAAGCAGCAACAGC
  AGCAGCCACCACCCCCATCTGAGCCAGCAGCCAGCAGCTCAGGCCGGCCAGCGGCCTCCCCTGGCACTTC
  GGAAGCAAGCAGCCAGGGCTCCGGGGAAGGAGAGGGCATCCAGCTGACCGCGGCTCAGGAGCTGATGATC
  CAGCAGTTAGTTGCCGCGCAGCTGCAGTGCAACAAACGATCTTTCTCCGACCAGCCCAAAGTCACGCCCT
  GGCCCCTGGGTGCAGACCCTCAGTCCCGAGATGCCCGTCAGCAACGCTTTGCCCACTTCACCGAGCTAGC
  CATCATCTCGGTCCAGGAGATTGTGGACTTTGCCAAGCAGGTGCCAGGGTTCTTGCAGTTGGGCCGGGAG
  GACCAGATCGCCCTCCTGAAGGCGTCCACCATTGAGATCATGTTGCTAGAAACAGCCAGACGCTACAACC
  ACGAGACAGAATGCATCACGTTCCTGAAGGACTTCACCTACAGCAAGGACGACTTCCACCGTGCAGGCTT
  GCAGGTGGAATTCATCAATCCCATCTTCGAGTTCTCGCGGGCCATGCGGCGGCTGGGCCTGGACGATGCA
  GAGTATGCCTTGCTTATCGCCATCAACATCTTCTCAGCCGATCGGCCTAATGTGCAGGAGCCCAGCCGTG
  TGGAGGCCCTGCAGCAGCCCTACGTGGAGGCGCTCCTCTCCTACACGAGGATCAAGCGCCCACAGGACCA
  GCTCCGCTTCCCACGCATGCTCATGAAGCTGGTGAGCCTGCGCACCCTCAGCTCCGTGCACTCGGAGCAG
  GTCTTTGCATTGCGACTCCAGGACAAGAAGCTGCCGCCCTTGCTGTCCGAGATCTGGGATGTGCACGAGT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285518
• Insert Size: 1332 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285518.1, NP_001272447.1
• RefSeq Size: 2016 bp
• RefSeq ORF: 1332 bp
• Locus ID: 22260
• UniProt ID: Q60644
• Cytogenetics: 7 B3
• Gene Summary: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:18055760, PubMed:19520913, PubMed:20427281). Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4) (PubMed:18055760, PubMed:19520913, PubMed:20427281). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8 (PubMed:18055760, PubMed:19520913, PubMed:20427281). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (PubMed:18055760, PubMed:19520913, PubMed:20427281). Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 3 and 4 both encode the same isoform (b).