Taf1a (NM_001277957) Mouse Untagged Clone

CAT#: MC227717

Taf1a (untagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A (Taf1a), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf1a
• Synonyms: mTAFI48; TAFI48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227717 representing NM_001277957
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGTGATTTCGGTGAAGAGTTGACAAAGCTGGCTGTGGCAGAGGATAACCCAGAAACGTCGGTGC
  TCTCTAAAACGGGAATGCATTTCCCATGGCTTCACAAGCACGTTGAAGCTGTAGTGACTGGAGGGAAGAA
  GAGGAAGGATTTTGCTCAGACGACAAGTGCTTGTTTAAGCTTTATCCAGGAAGCCCTGCTGAAGCACCAG
  TGGCAGCAAGCTGCAGAGTATATGCACAGTTACCTGCAGACCCTGGAGGACTCAGACACCGACAAGAGAC
  AGGCTGCCCCTGAGATCATTTGGAAGCTTGGAAGTGAAATTCTATTTTACCACCCCAAAAGCAATGTGGA
  GACTTTTAATAGCTTCGCTGACCGGATGAAAAATATTGGTGTCTTGAATTATTTAAAGATCTCCTTACAA
  CACGCCTTGTATCTTTTACATCATGGAATGCTTGATGATGCGAACAGGAACCTGAGTAAGGCAGAGACGT
  GGAGGTATGGAGAAAAGTCGTCTTCTCAGGAAGTGTTGATCAACCTTGTTCAGGCTTACAAAGGGCTTCT
  GCAGTACTACACTTGGACCAGAAAGAAGATGGAGTTGTCAAAGCTTGATGAAGATGATTACGCTTACGCC
  GCCAAAACCCGAACCATGCTCAGCCAGAGCTGCAAGACGTCTACAAATATCTGTGCACTGGTCAAAACCC
  CCGGGGTCTGGGATCCTTTTGTGAAGAGTTATGTGGAGATGCTGGAGTTCTATGGTGATCAAGATGGAGC
  CCGAGAGATGCTCACCAATTATGCATATGATGAGAAGTTCCCGTCGAACCCCAACGCCCACGTCTACTTA
  TACGAGTTTCTCAAGAGAGAGAAGGCACCGAGGGCGAAGCTCATAAGTGTTCTTAAGATTTTACATGAGA
  TTGTGCCATCCCATACACTAATGTTAGAATTCCATACATTGCTTAGAAAATCAGACACAGAAGAACACCA
  GAAACTGGGCTTGTCTGTGTTATTTGAAGTCCTGGACTTCGCTGGATGCAATAAGAACATAACGGCCTGG
  AAATACTTGGCAAAGTATCTGAAGCAGATATTAGTGGGAAGCCATCACGAGTGGGTTGAAGAAGAGTGGA
  AGTCTAGGAGGAACTGGTGGCCAGCTTTTCACTTCAGCTTCTTTTGGGCGAAGAGTGACTGGAAGGCAGA
  CACTGACTTGGCTTGTTGTAAATATTTTCGGTATATTTTAAAACAAGATCATGAAACCTTGAAGAAGAAA
  ATCAAGCGAATGAAGAAGTCTGTGAAAAAATACACTATTGTAAATCCAGGTGTTCACACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277957
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277957.1, NP_001264886.1
• RefSeq Size: 2391 bp
• RefSeq ORF: 1323 bp
• Locus ID: 21339
• UniProt ID: P97357
• Cytogenetics: 1 H5
• Gene Summary: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.