Erg (NM_001302183) Mouse Untagged Clone

CAT#: MC227711

Erg (untagged) - Mouse avian erythroblastosis virus E-26 (v-ets) oncogene related (Erg), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erg
• Synonyms: D030036I24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227711 representing NM_001302183
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCAGACTGTACCTGACCCAGCAGCTCATATTAAGGAGGCCTTGTCAGTTGTGAGCGAGGACCAGT
  CACTATTTGAGTGTGCCTACGGAACGCCACACCTGGCTAAGACAGAGATGACCGCATCCTCTTCCAGTGA
  CTATGGCCAGACATCCAAGATGAGTCCCAGAGTCCCTCAGCAGGACTGGCTGTCTCAAGCCCCAGCCAGG
  GTCACCATCAAGATGGAGTGCAACCCTAGTCAGGTGAATGGTTCCAGGAACTCACCTGATGAGTGCAGTG
  TGAACAAAGGTGGGAAGATGGTGGGCAGCCCGGATACTGTGGGGATGAGCTACGGCAGCTACATGGAGGA
  GAAGCATGTGCCGCCTCCCAATATGACCACAAATGAGCGCAGAGTGATCGTCCCTGCAGATCCTACTCTG
  TGGAGCACAGACCATGTCCGACAGTGGCTGGAGTGGGCGGTGAAAGAATATGGCCTCCTCGATGTGGACG
  TCTTACTATTTCAGAATATCGATGGGAAGGAGCTGTGCAAGATGACAAAGGATGACTTCCAGCGGCTCAC
  GCCGAGCTACAATGCCGACATTCTTCTCTCACATCTCCACTACCTCAGAGAGACTCCCCTTCCACATCTG
  ACTTCCGATGACGTTGATAAGGCTTTACAAAACTCTCCACGGTTAATGCATGCCAGAAACACAGCTGCTC
  AGCCATCTCCCTCTGCAGTGCCCAAAACTGAAGACCAGCGTCCTCAGTTAGATCCTTACCAGATCCTGGG
  ACCGACCAGTAGCCGCCTTGCTAATCCAGGTAGTGGCCAGATCCAGCTGTGGCAGTTCCTGCTCGAACTC
  CTGTCAGACAGCTCCAACTCCAACTGCATCACCTGGGAAGGCACCAACGGGGAGTTCAAGATGACAGACC
  CGGACGAGGTGGCTCGGCGCTGGGGGGAGAGGAAGAGCAAGCCCAACATGAACTATGACAAGCTCAGCCG
  CGCCCTCCGCTACTACTACGACAAAAACATCATGACCAAGGTGCACGGGAAGCGCTACGCCTACAAGTTT
  GACTTCCACGGGATTGCCCAGGCCCTGCAGCCCCACCCTCCTGAGTCGTCCCTGTACAAGTACCCCTCCG
  ACCTGCCATACATGGGCTCCTATCACGCCCACCCCCAGAAGATGAACTTTGTGTCTCCCCACCCTCCCGC
  TCTCCCAGTCACATCTTCCAGTTTCTTTGCTTCCCCGAACCCATACTGGAATTCACCGACTGGGGGCATC
  TACCCGAACACTAGGCTCCCAGCCAGCCATATGCCCTCTCACCTGGGCACCTACTACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302183
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302183.1, NP_001289112.1
• RefSeq Size: 3118 bp
• RefSeq ORF: 1320 bp
• Locus ID: 13876
• UniProt ID: P81270
• Cytogenetics: 16 56.04 cM
• Gene Summary: Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (6) is shorter compared to isoform 1.