Osbpl1a (NM_001252493) Mouse Untagged Clone

CAT#: MC227702

Osbpl1a (untagged) - Mouse oxysterol binding protein-like 1A (Osbpl1a), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Osbpl1a
• Synonyms: G430090F17Rik; Gm753; ORP-1; Osbpl1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227702 representing NM_001252493
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAAGGAAAAGACTGTGGTGGGGGAGATGCGCTCTCCAATGGCATCAAAAAGCACAGAACAAGCC
  TGCCATCACCTATGTTCTCCAGAAACGATTTCAGCATCTGGAGCATCCTCAGAAAATGCATTGGGATGGA
  ACTGTCCAAGATCACAATGCCAGTGATATTTAATGAGCCTCTGAGCTTCCTGCAGCGGCTAACTGAATAC
  ATGGAGCACACGTACCTCATCCACAAAGCCAGTTCACTTTCTGATCCTGTGGAAAGGATGCAGTGTGTGG
  CTGCATTTGCTGTGTCTGCTGTCGCCTCTCAGTGGGAGCGCACCGGAAAGCCCTTCAACCCGCTTCTGGG
  AGAGACTTATGAATTAGTTCGAGATGACCTTGGGTTTAGGCTCATCTCAGAACAGGTCAGCCATCATCCC
  CCAATCAGTGCATTCCATGCAGAAGGGCTCAACAATGACTTCATCTTCCATGGCTCAATTTACCCCAAAC
  TGAAGTTCTGGGGCAAGAGTGTAGAAGCAGAGCCTAAAGGAACCATCACCTTGGAGCTTTTAGACCACAA
  CGAAGCATACACATGGACAAACCCCACCTGTTGTGTGCATAACATCATCGTGGGCAAGCTCTGGATTGAA
  CAGTATGGCAACGTGGAAATCATAAACCACAAGACTGGGGACAAATGTGTGCTGAATTTTAAGCCATGTG
  GTCTTTTTGGCAAGGAATTACACAAAGTTGAAGGCTACATACAAGATAAAAGCAAAAAGAAGCTCTGTGC
  TCTCTATGGGAAGTGGACTGAGTGCTTGTACAGCGTAGACCCTGCCACTTTTGATGCTTACAAAAAAAAT
  GATAAGAAAAACACAGAAGAGAAGAAGAACAGCAAACAGACAAGCTCATCTGAGGAGTCTGATGAAATGC
  CAGTGCCAGATTCCGAGAGCGTATTCATTATCCCTGGAAGTGTGCTCCTGTGGCGGATAGCCCCGCGGCC
  TCCCAACTCTGCTCAGATGTATAACTTTACCAGCTTTGCCATGGTTTTGAATGAAGTCGACAAGGAGATG
  GAGAGTGTGATTCCCAAGACTGACTGCAGGCTACGGCCTGACATCAGAGCCATGGAGAACGGAGAGATAG
  ATCTAGCTAGTGAAGAAAAGAAACGGCTTGAGGAAAAGCAAAGAGCAGCCCGCAAGAACAGGTCCAAGTC
  GGAAGAGGACTGGAAGACAAGGTGGTTCCATCAAGGTCCTAACCCCTACAGTGGAGCACAGGACTGGATT
  TATTCTGGCAGCTACTGGGACAGAAACTACTTCAATTTGCCTGATATTTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252493
• Insert Size: 1314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252493.1, NP_001239422.1
• RefSeq Size: 2980 bp
• RefSeq ORF: 1314 bp
• Locus ID: 64291
• UniProt ID: Q91XL9
• Cytogenetics: 18 A1
• Gene Summary: Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) differs in the 5' UTR and 3' UTR, lacks a large portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 3, 4, 5 and 6 encode the same isoform (3), which has a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.