Pax6 (NM_001244200) Mouse Untagged Clone

CAT#: MC227695

Pax6 (untagged) - Mouse paired box 6 (Pax6), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pax6
• Synonyms: 1500038E17Rik; AEY1; AEY11; Dey; Gsfaey; Gsfaey11; Pax; Pax-6; Sey
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227695 representing NM_001244200
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAACAGTCACAGCGGAGTGAATCAGCTTGGTGGTGTCTTTGTCAACGGGCGGCCACTGCCGGACT
  CCACCCGGCAGAAGATCGTAGAGCTAGCTCACAGCGGGGCCCGGCCGTGCGACATTTCCCGAATTCTGCA
  GACCCATGCAGATGCAAAAGTCCAGGTGCTGGACAATGAAAACGTATCCAACGGTTGTGTGAGTAAAATT
  CTGGGCAGGTATTACGAGACTGGCTCCATCAGACCCAGGGCAATCGGAGGGAGTAAGCCAAGAGTGGCGA
  CTCCAGAAGTTGTAAGCAAAATAGCCCAGTATAAACGGGAGTGCCCTTCCATCTTTGCTTGGGAAATCCG
  AGACAGATTATTATCCGAGGGGGTCTGTACCAACGATAACATACCCAGTGTGTCATCAATAAACAGAGTT
  CTTCGCAACCTGGCTAGCGAAAAGCAACAGATGGGCGCAGACGGCATGTATGATAAACTAAGGATGTTGA
  ACGGGCAGACCGGAAGCTGGGGCACACGCCCTGGTTGGTATCCCGGGACTTCAGTACCAGGGCAACCCAC
  GCAAGATGGCTGCCAGCAACAGGAAGGAGGGGGAGAGAACACCAACTCCATCAGTTCTAACGGAGAAGAC
  TCGGATGAAGCTCAGATGCGACTTCAGCTGAAGCGGAAGCTGCAAAGAAATAGAACATCTTTTACCCAAG
  AGCAGATTGAGGCTCTGGAGAAAGAGTTTGAGAGGACCCATTATCCAGATGTGTTTGCCCGGGAAAGACT
  AGCAGCCAAAATAGATCTACCTGAAGCAAGAATACAGGTATGGTTTTCTAATCGAAGGGCCAAATGGAGA
  AGAGAAGAGAAACTGAGGAACCAGAGAAGACAGGCCAGCAACACTCCTAGTCACATTCCTATCAGCAGCA
  GCTTCAGTACCAGTGTCTACCAGCCAATCCCACAGCCCACCACACCTGTCTCCTCCTTCACATCAGGTTC
  CATGTTGGGCCGAACAGACACCGCCCTCACCAACACGTACAGTGCTTTGCCACCCATGCCCAGCTTCACC
  ATGGCAAACAACCTGCCTATGCAACCCCCAGTCCCCAGTCAGACCTCCTCATACTCGTGCATGCTGCCCA
  CCAGCCCGTCAGTGAATGGGCGGAGTTATGATACCTACACCCCTCCGCACATGCAAACACACATGAACAG
  TCAGCCCATGGGCACCTCGGGGACCACTTCAACAGGACTCATTTCACCTGGAGTGTCAGTTCCCGTCCAA
  GTTCCCGGGAGTGAACCTGACATGTCTCAGTACTGGCCTCGATTACAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001244200
• Insert Size: 1311 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244200.2, NP_001231129.1
• RefSeq Size: 3726 bp
• RefSeq ORF: 1311 bp
• Locus ID: 18508
• UniProt ID: P63015
• Cytogenetics: 2 55.31 cM
• Gene Summary: This gene encodes a homeobox-containing protein that functions as a regulator of transcription. It plays a key role in the development of neural tissues, particularly the eye. Activity of this protein is also required for expression of glucagon in the pancreas. This gene is regulated by multiple enhancers located up to tens or hundreds of kilobases upstream and downstream of the transcription start sites. Mutations in this gene or deletion of these regulatory elements results in severe defects in eye development. Alternative splicing and the use of alternative promoters results in multiple transcript variants, some of which encode proteins that lack the N-terminal paired domain. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. It initiates from the P1 promoter. Variants 1, 2, and 3 encode the same protein (isoform 1, also known as 5a).