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Sept7 (NM_001205367) Mouse Untagged Clone

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SKU
MC227693
42254 (untagged) - Mouse septin 7 (Sept7), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $503.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sept7
Synonyms Cdc10; E430034N22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227693 representing NM_001205367
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGCACCATGGCTCAACCGA
AGAACCTTGAGGGCTATGTGGGCTTTGCCAACCTCCCAAATCAAGTGTACAGAAAATCCGTGAAAAGAGG
ATTTGAATTCACTCTTATGGTAGTAGGTGAATCTGGACTGGGAAAGTCGACATTAATCAACTCATTATTC
CTCACAGATTTGTATTCTCCAGAGTATCCAGGACCTTCTCATAGAATCAAAAAGACTGTACAGGTGGAGC
AATCCAAAGTTTTAATCAAAGAAGGTGGTGTTCAGTTGCTGCTGACAATAGTTGATACTCCAGGATTTGG
AGATGCAGTGGATAATAGTAATTGCTGGCAGCCTGTTATCGACTACATTGATAGTAAATTTGAAGATTAC
TTAAATGCAGAATCTCGAGTGAACAGACGTCAGATGCCTGATAACAGGGTGCAGTGTTGTTTATACTTCA
TTGCTCCTTCAGGACATGGACTTAAACCATTGGACATTGAGTTTATGAAACGTTTGCATGAAAAAGTGAA
TATCATCCCATTAATTGCCAAAGCAGACACACTTACACCAGAGGAATGCCAACAGTTTAAAAAGCAGATA
ATGAAAGAAATTCAAGAACATAAAATTAAAATATATGAATTTCCAGAAACAGATGATGAAGAAGAGAATA
AACTGGTTAAGAAGATAAAGGACCGTTTACCTCTTGCTGTGGTAGGTAGTAATACTATCATTGAAGTTAA
TGGCAAAAGAGTCAGAGGAAGGCAGTATCCTTGGGGTGTGGCTGAAGTTGAAAATGGTGAACATTGTGAT
TTCACAATTCTAAGAAATATGTTGATAAGAACACATATGCAGGACTTGAAAGATGTTACCAATAACGTAC
ACTATGAGAACTACAGAAGCAGAAAGCTAGCAGCAGTGACTTACAATGGAGTGGATAACAACAAGAACAA
AGGGCAGCTTACCAAGAGCCCTCTGGCACAGATGGAAGAAGAAAGAAGGGAACATGTAGCCAAAATGAAG
AAGATGGAGATGGAGATGGAACAGGTGTTTGAGATGAAGGTCAAAGAAAAAGTTCAAAAACTGAAGGACT
CTGAAGCAGAGCTCCAGCGGCGCCATGAGCAAATGAAAAAGAATTTAGAAGCACAGCACAAAGAATTAGA
GGAAAAACGTCGTCAGTTTGAAGAAGAAAAGGCAAACTGGGAAGCTCAACAGCGTATTTTAGAGCAACAG
AACTCTTCAAGAACCTTGGAAAAGAACAAGAAGAAAGGCAAGATCTTTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001205367
Insert Size 1311 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001205367.1, NP_001192296.1
RefSeq Size 2530 bp
RefSeq ORF 1311 bp
Locus ID 235072
UniProt ID O55131
Cytogenetics 9 A4
Summary Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001205367" in other vectors (1)
SKU Description Size Price
MR229904 42254 (myc-DDK-tagged) - Mouse septin 7 (Sept7), transcript variant 2 10 ug
$289.00 $457.00

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