Uba3 (NM_001301858) Mouse Untagged Clone

CAT#: MC227687

Uba3 (untagged) - Mouse ubiquitin-like modifier activating enzyme 3 (Uba3), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uba3
• Synonyms: A830034N06Rik; AI256736; AI848246; AW546539; Ube1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227687 representing NM_001301858
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGGCGAGGAGCCGGAGAAGAAAAGAAGGAGAATAGAGGAGCTGCTGGCTGAGAAAATGGCTG
  TTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGGAACCATGTAAAGAAGTTCCTCGAGCG
  GTCTGGACCCTTCACACACCCCGATTTCGAACCAAGCACTGAAGCATTATCTGGTTTTAGACAGATTCAT
  GTTATAGACATGGACACTATAGATGTTTCCAATTTAAATAGACAGTTTTTATTTAGGCCTAAAGATGTCG
  GAAGACCCAAGGCTGAAGTTGCTGCAGAATTCCTAAATGACAGAGTTCCTAACTGCAACGTGGTACCACA
  TTTCAACAAGATACAAGATTTTAACGACACTTTCTACCGACAATTTCATATCATTGTATGTGGCCTGGAC
  TCTATCATAGCGAGAAGATGGATCAATGGAATGCTGATATCTCTTCTAAATTATGAAGATGGTGTGTTGG
  ATCCAAGCTCCATTGTACCTTTGATAGATGGGGGGACAGAAGGCTTTAAAGGGAATGCCCGAGTGATTTT
  GCCTGGAATGACCGCTTGTATTGAGTGCACTCTGGAACTTTACCCACCACAGGTCAATTTCCCCATGTGT
  ACCATTGCATCTATGCCCAGGCTCCCAGAACACTGTATCGAGTATGTGAGGATGTTGCAATGGCCTAAAG
  AGCAGCCTTTTGGAGATGGGGTTCCATTAGATGGAGATGACCCTGAACATATTCAGTGGATTTTCCAAAA
  GTCCATAGAGAGAGCATCACAATATAATATTAGAGGCGTTACCTACAGACTCACTCAAGGGGTGGTAAAA
  CGAATCATTCCTGCAGTAGCTTCTACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGAGGTTTTCAAGA
  TAGCTACAAGTGCGTACATTCCCCTTAATAACTACCTGGTATTCAATGATGTAGATGGGCTGTACACTTA
  CACGTTTGAAGCAGAGAGAAAGGAAAACTGTCCAGCATGTAGCCAACTTCCTCAAAACATTCAGTTTTCC
  CCATCAGCTAAACTACAGGAGGTCTTAGACTACCTAACCAACAGTGCTTCTCTGCAAATGAAGTCTCCGG
  CTATCACAGCCACATTAGAGGGGAAGAACAGGACACTTTACTTACAGTCAGTAACGTCTATTGAAGAACG
  AACCAGGCCCAATCTTTCCAAAACATTAAAAGAACTGGGACTAGTTGATGGACAAGAACTGGCTGTTGCT
  GATGTCACTACACCACAGACTGTACTATTCAAACTTCATTTTACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301858
• Insert Size: 1308 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301858.1, NP_001288787.1
• RefSeq Size: 2551 bp
• RefSeq ORF: 1308 bp
• Locus ID: 22200
• UniProt ID: Q8C878
• Cytogenetics: 6 D3
• Gene Summary: The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes an isoform (4) that is shorter compared to isoform 1.