Rcc1 (NM_001197082) Mouse Untagged Clone

CAT#: MC227676

Rcc1 (untagged) - Mouse regulator of chromosome condensation 1 (Rcc1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rcc1
• Synonyms: 4931417M11Rik; AI326872; Chc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227676 representing NM_001197082
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCAAGCGCATAGCTAAGAGAAGGTCACCCCCAGAAGATGCCATCCCCAAAAGCAAGAAGGTGA
  AAGACACTCGTAATCGCGGTCCTGCTACTCGCTCGTGCCAAGTCTCACACAGGTCCCACAACACAGAACC
  AGGCTTGGTGCTGACACTGGGCCAGGGCGACGTGGGCCAGCTGGGGCTGGGTGAAAGTGTGCTGGAGAGG
  AAGAAGCCGGCCTTGGTGCCCCTTCTGCAGGATGTTGTGCAGGCTGAGGCCGGGGGCATGCATACTGTGT
  GTCTGAGCCAAAGTGGCCAGGTCTACTCCTTTGGCTGCAATGACGAGGGTGCCCTGGGAAGGGACACTTC
  AGTCGAAGGCTCAGAAATGGTCCCTGGGAAAGTGGAACTGCAAGAGAAAGTGGTGCAAGTGTCAGCGGGG
  GACAGTCACACAGCAGCCCTCACCGAAGACGGCCGTGTCTTCCTTTGGGGCTCTTTCCGGGACAATAATG
  GTGTGATCGGGTTGTTGGAGCCCATGAAGAAGAGCATGGTACCTGTTCAAGTGCAGCTGGATGCGCCCGT
  GGTAAAGGTGGCCTCGGGGAACGACCACTTGGTGATGCTCACGAACGATGGCGACCTCTACACCTTGGGT
  TGTGGGGAGCAAGGTCAGCTGGGCCGTGTACCTGAATTATTTGCCAACCGAGGTGGCCGTCAGGGCCTTG
  GGCGACTCCTGGTCCCCAGATGTGTGCTGCTGAAATCCAGGGGGACCCGGGGCCGTGTGCGGTTCCAGGA
  TGCCTTCTGTGGCGCCTATTTCACTTTTGCCATCTCCCGAGAGGGCCACGTGTATGGCTTTGGCCTCTCC
  AACTATCACCAGCTCGGGACTCCAGGCACTGGATCTTGCTTCATTCCTCAAAACTTGACATCCTTCAAGA
  ATTCCACCAAGTCCTGGGTAGGCTTCTCCGGTGGCCAGCATCACACAGTCTGCATGGATTCGGAAGGGAA
  GGCGTACAGCCTGGGCCGGGCTGAGTACGGGCGCCTGGGCCTTGGGGAGGGTGCTGAGGAGAAGAGCATA
  CCCACCCTCATTTCCCGGCTGCCCGTGGTCTCCTCAGTGGCCTGTGGGGCTTCTGTGGGTTATGCTGTGT
  CCAAGGATGGTCGTGTTTTTGCCTGGGGCATGGGCACCAACTACCAGCTGGGCACCGGGCAGGATGAAGA
  TGCCTGGAGTCCTGTGGAAATGACTGGCAAACAGCTGGAGAACCGTGTGGTGTTAACTGTGTCCAGTGGG
  GGCCAGCACACGGTCTTACTAGTTAAGGACCAGGCACAGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001197082
• Insert Size: 1305 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001197082.1, NP_001184011.1
• RefSeq Size: 2332 bp
• RefSeq ORF: 1305 bp
• Locus ID: 100088
• Cytogenetics: 4 D2.3
• Gene Summary: Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).