Trpc4 (NM_001253683) Mouse Untagged Clone

CAT#: MC227667

Trpc4 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 (Trpc4), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpc4
• Synonyms: CCE1; STRPC4; Trp4; Trrp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227667 representing NM_001253683
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTTCTATTACAAAAGAAATGTCAACGCCCCCTACAGAGACCGCATCCCACTGAGGATTGTCA
  GAGCAGAATCTGAGCTCTCACCATCAGAGAAAGCCTACTTGAATGCTGTGGAGAAGGGGGACTATGCAAG
  CGTCAAGAAGTCTCTGGAGGAAGCTGAGATTTATTTTAAAATCAACATTAACTGCATCGACCCCCTGGGA
  AGGACCGCCCTCCTCATTGCCATTGAAAATGAGAATCTGGAGCTTATTGAACTATTGTTGAGTTTCAATG
  TCTATGTAGGCGATGCGCTGCTTCACGCCATCAGAAAAGAGGTGGTTGGAGCCGTGGAGCTACTGCTGAA
  CCACAAAAAGCCAAGTGGAGAGAAGCAGGTGCCTCCCATTCTCCTTGATAAACAGTTCTCTGAATTCACT
  CCGGACATCACACCCATCATCTTGGCTGCACATACAAATAATTACGAGATAATCAAACTTTTGGTTCAGA
  AAGGTGTCTCAGTGCCCAGACCCCACGAGGTCCGCTGTAACTGTGTTGAGTGTGTCTCCAGCTCGGATGT
  GGACAGCCTCAGGCATTCACGGTCCAGGCTCAACATCTACAAGGCCTTGGCCAGCCCCTCGCTCATTGCC
  CTGTCAAGCGAAGACCCTTTCCTTACTGCCTTTCAGTTAAGTTGGGAGCTGCAAGAACTCAGCAAGGTGG
  AGAACGAATTCAAGTCGGAGTATGAGGAGCTGTCTAGACAGTGCAAACAATTTGCCAAGGACCTCCTAGA
  TCAGACACGGAGTTCCAGAGAGCTGGAAATCATTCTTAATTACCGTGATGACAATAGTCTGATCGAAGAA
  CAGAGTGGAAATGATCTTGCAAGGCTAAAATTAGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCTCAGC
  CCAACTGCCAGCAGCTGCTCGCTTCCCGCTGGTACGATGAGTTCCCAGGCTGGAGGAGAAGACACTGGGC
  GGTGAAGATGGTGACGTGTTTCATAATAGGACTACTCTTCCCCGTCTTCTCCGTGTGCTACCTGATAGCT
  CCCAAAAGCCCACTTGGACTGTTCATCAGAAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATC
  TGACCTTTTTGTTTCTGCTGCTGCTAGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC
  ACCACCAACCATCGTGGAGTGGATGATATTACCGTGGGTCCTGGGTAAATGTGTTACTCTAGAAAATATA
  ATAACGAACGTGTCTCTGCCGTGGAATTTTGTTGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253683
• Insert Size: 1299 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253683.1, NP_001240612.1
• RefSeq Size: 2986 bp
• RefSeq ORF: 1299 bp
• Locus ID: 22066
• Cytogenetics: 3 25.43 cM
• Gene Summary: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus compared to isoform 1.