Sept8 (NM_001252332) Mouse Untagged Clone

CAT#: MC227656

42255 (untagged) - Mouse septin 8 (Sept8), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sept8
• Synonyms: AW046166; Sepl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227656 representing NM_001252332
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCACCGACTTGGAACGCGTCTCGAACGCAGAGCCTGAGCCCCGGAGCCTGTCCCTGGGCGGCC
  ATGTTGGGTTTGACAGCCTCCCTGACCAGCTGGTCAGCAAGTCAGTCACTCAGGGCTTCAGCTTCAACAT
  ACTCTGTGTGGGCGAAACTGGGATTGGCAAGTCCACGCTAATGAACACACTCTTCAACACGACCTTTGAG
  ACTGAAGAAGCCAGTCACCATGAAGAGTGTGTGCGCCTGCGGCCTCAGACCTATGACCTCCAAGAGAGTA
  ACGTTCATCTCAAGCTGACCATCGTGGATGCTGTGGGCTTTGGCGATCAGATCAATAAGGATGACAGTTA
  CAGGCCCATAGTTGACTACATCGACGCGCAGTTTGAAAACTATCTGCAGGAGGAGTTGAAGATCCGCCGT
  TCCCTCTTTGACTATCACGACACGAGGATCCATGTTTGCCTCTACTTCATCACGCCCACCGGGCACTCCC
  TGAAGTCCCTGGATCTGGTGACCATGAAGAAACTAGATAGCAAGGTGAATATAATCCCCATCATTGCCAA
  GGCTGACACCATCTCCAAGAGCGAGCTCCACAAGTTCAAAATCAAGATCATGGGTGAGCTGGTCAGCAAC
  GGAGTCCAGATCTACCAGTTTCCCACCGATGACGAGGCTGTCGCCGAGATTAATGCAGTCATGAACGCAC
  ACCTGCCTTTTGCCGTGGTGGGCAGCACAGAGGAGGTGAAGGTGGGGAATAAGCTGGTTCGAGCACGACA
  GTACCCTTGGGGTGTGGTGCAGGTGGAGAATGAGAATCACTGTGACTTCGTGAAGCTGCGGGAGATGCTG
  ATCCGGGTGAACATGGAGGACCTGCGCGAGCAGACCCACAGCCGGCACTACGAGCTCTACCGCCGCTGCA
  AGTTGGAGGAGATGGGCTTCCAGGACAGTGACGGTGACAGCCAGCCCTTCAGCCTCCAAGAGACATATGA
  GGCAAAGAGGAAGGAGTTCCTAAGCGAGCTGCAGAGGAAGGAGGAAGAGATGCGACAGATGTTTGTCAAC
  AAAGTCAAGGAGACAGAGCTTGAGCTGAAGGAGAAGGAACGGGAGCTCCATGAGAAGTTTGAGCACCTGA
  AGCGAATCCACCAGGAGGAGAAGCGCAAGGTAGAGGAGAAGCGCAGGGAGCTGGAAGAGGAGACCAACGC
  CTTCAACTGCCGGAAGGCGGCCATGGAGGCCCTGCAGTCACAGGCCTTGCATGCCACTTCACAGCAGCCT
  CTGAGGAAAGACAAGGACAAGAAGAATTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001252332
• Insert Size: 1290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252332.1, NP_001239261.1
• RefSeq Size: 4467 bp
• RefSeq ORF: 1290 bp
• Locus ID: 20362
• UniProt ID: Q8CHH9
• Cytogenetics: 11 31.96 cM
• Gene Summary: Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (By similarity). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate 3' terminal exon, resulting in novel 3' coding region and 3' UTR, compared to variant 4. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 4.