Kcnj12 (NM_001267593) Mouse Untagged Clone

CAT#: MC227642

Kcnj12 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnj12
• Synonyms: IRK-2; IRK2; Kir2.2; MB-IRK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227642 representing NM_001267593
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCAGCCAGTCGGGCCAACCCCTACAGCATCGTATCATCAGAGGAGGACGGGCTGCACCTGGTTA
  CCATGTCAGGCGCCAACGGTTTTGGCAATGGCAAGGTGCATACACGGCGCCGGTGCCGCAACCGCTTCGT
  CAAGAAGAACGGTCAGTGCAACATTGAATTCGCCAACATGGACGAGAAGTCACAACGCTACCTGGCTGAC
  ATGTTTACCACGTGTGTGGACATCCGCTGGCGCTACATGCTGCTCATCTTCTCTCTGGCCTTTCTTGCCT
  CCTGGTTGTTGTTTGGCATCATCTTCTGGGTCATTGCTGTCGCCCACGGGGACCTGGAGCCAGCCGAGGG
  CCGTGGCCGTACACCCTGTGTGCTGCAGGTCCACGGCTTCATGGCAGCCTTTCTCTTCTCCATTGAGACA
  CAGACCACCATTGGCTACGGGCTACGCTGTGTGACTGAAGAGTGCCCGGTGGCTGTCTTCATGGTGGTGG
  CGCAGTCCATTGTGGGCTGCATCATTGACTCCTTCATGATTGGTGCCATCATGGCCAAGATGGCACGGCC
  CAAGAAGCGCGCACAGACTCTGCTTTTCAGCCATAATGCCGTGGTGGCTCTGCGTGACGGCAAGCTCTGC
  CTCATGTGGCGCGTGGGCAACCTGCGTAAGAGTCACATCGTGGAGGCCCATGTGCGGGCCCAGCTCATCA
  AGCCCAGGGTCACAGAGGAGGGTGAGTACATCCCACTGGACCAGATTGACATCGATGTCGGCTTTGACAA
  GGGCCTAGACCGTATCTTCCTGGTATCACCCATCACCATCTTGCACGAGATTGATGAGGCCAGCCCACTG
  TTTGGCATTAGCCGTCAGGACCTTGAGACAGACGACTTTGAGATTGTGGTCATCCTGGAGGGCATGGTAG
  AGGCCACAGCCATGACCACACAGGCTCGCAGTTCCTACCTGGCTAACGAGATCCTGTGGGGCCACCGCTT
  TGAGCCAGTGCTCTTCGAAGAGAAGAACCAGTACAAGATTGACTATTCACACTTCCACAAGACCTACGAG
  GTGCCATCTACACCCCGCTGCAGCGCCAAGGACCTGGTGGAGAACAAGTTCCTCCTGCCCAGCGCCAACT
  CTTTCTGCTATGAGAACGAGCTGGCCTTCCTGAGCAGAGATGAGGAGGACGAGGTGGCTACCGACCGGGA
  TGGCCGCAGCCCTCAGCCCGAGCATGACTTTGACAGACTGCAGGCCAGCAGCGCTGCCCTTGAACGGCCC
  TACAGACGGGAGTCGGAGATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001267593
• Insert Size: 1284 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267593.1, NP_001254522.1
• RefSeq Size: 4427 bp
• RefSeq ORF: 1284 bp
• Locus ID: 16515
• UniProt ID: P52187
• Cytogenetics: 11 37.96 cM
• Gene Summary: Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.