Syt6 (NM_001276677) Mouse Untagged Clone

CAT#: MC227631

Syt6 (untagged) - Mouse synaptotagmin VI (Syt6), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Syt6
• Synonyms: 3110037A08Rik; AW048930; sytVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227631 representing NM_001276677
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGGAGGAAGAAAGAGGCCTCCAGCCCTTCTTCTGCTAACCCTGCTTCAGAGACTCTCCAGAGCC
  CCAGCTCCAGAGGCAACATGGCGGATAAGCTGAAGGACCCCAGTGCCCTGGGCTTCCTGGAGGCAGCTGT
  GAAGATCAGCCACACTTCCCCAGACATCCCAGCCGAGGTGCAGATGTCGGTCAAAGAGCACATCATGCGC
  CACACAAAGCTGCAGCGACAGACCACAGAGCCAGCATCCTCCACCAGGCACACATCCTTCAAGCGCCACC
  TGCCACGACAGATGCATGTCTCCAGCGTGGACTATGGCAATGAGCTGCCGCCGGCTGCCGCCGAGCAGCC
  CACCAGTATTGGCCGTATCAAGCCTGAGCTTTACAAGCAGAAGTCAGTGGATGGGGATGATGCCAAGTCG
  GAGGCCGCCAAGAGCTGTGGGAAGATCAACTTCAGCCTCCGCTATGACTATGAAAGCGAGACGCTGATTG
  TGCGCATCCTGAAGGCCTTTGACCTCCCTGCCAAGGACTTTTGCGGAAGTTCTGACCCTTATGTTAAGAT
  CTACCTCCTGCCTGACCGCAAGTGCAAGCTGCAGACCCGGGTGCACCGCAAGACCCTGAACCCCACCTTT
  GATGAGAACTTCCACTTCCCCGTGCCCTACGAGGAGCTGGCTGACCGCAAGCTGCATCTCAGTGTCTTTG
  ACTTTGACCGCTTCTCCCGCCATGACATGATTGGGGAGGTCATCCTGGACAATCTCTTTGAGGCCTCTGA
  CCTGTCCCGGGAGACCTCCATCTGGAAGGACATCCAGTACGCTACTAGTGAAAGTGTGGACTTGGGAGAG
  ATCATGTTCTCCCTCTGCTACCTGCCCACAGCAGGCAGGCTCACCCTCACAGTGATCAAGTGTCGAAATC
  TCAAAGCAATGGACATCACAGGCTACTCAGATCCCTACGTCAAAGTATCCCTACTGTGTGATGGACGGAG
  GCTGAAAAAGAAGAAAACGACCATAAAAAAGAACACCCTAAACCCCATCTACAACGAGGCCATCATCTTT
  GACATCCCCCCAGAAAACATGGACCAAGTGAGCCTGCTCATCTCCGTTATGGACTATGATAGAGTTGGCC
  ACAACGAGATCATAGGAGTCTGTAGAGTGGGGATCAATGCTGAAGGCCTTGGCAGGGACCACTGGAATGA
  GATGTTGGCGTACCCACGAAAGCCCATTGCGCACTGGCACTCCTTGGTGGAGGTAAAGAAATCCTTCAAA
  GAGGGGACCCCTCGGTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276677
• Insert Size: 1281 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276677.1, NP_001263606.1
• RefSeq Size: 1665 bp
• RefSeq ORF: 1281 bp
• Locus ID: 54524
• Cytogenetics: 3 F2.2
• Gene Summary: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (By similarity). May mediate Ca(2+)-regulation of exocytosis in acrosomal reaction in sperm (PubMed:15774481).[UniProtKB/Swiss-Prot Function]