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Fam53b (NM_175268) Mouse Untagged Clone

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SKU
MC227618
Fam53b (untagged) - Mouse family with sequence similarity 53, member B (Fam53b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Fam53b
Synonyms A930008G19Rik; mKIAA0140
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227618 representing NM_175268
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGATGATCCTAAGGAGAAGCCTCATCAACCAGGGAGCTGACTCTGGAGCACACAGGACCTTCATCC
CTGAACTGCACACGCCAAAGATGAGTCAAGGACCTACCCTCCTTTCCTGTGGAATTATGGAAAATGACAG
ATGGCGAGACCTGGATAGAAAATGCCCTCTTCAGATTGACCAGCCAAGCGCCAGCATCTGGGAATGTCTG
CCTGAAAAGTGTCAGGATGGCTCCCTGTGGCACCAGGAGGCAGTGACTGCCTGTGCGGTGACCAGCCTAA
TCAAAGACCTCAACATAAATGACCACAATGGGAACCCCTCAGCTCCTCCCAGCAAACGCCAGTGCCGGTC
ACTATCCTTCTCTGATGAGATGTCCAGTTGCCGGACGTCATGGCGGCCCTTGGGGTCCAAAGTCTGGACT
CCCGTGGAGAAGAGACGATGCTACAGCGGAGGCAGCGTCCAGCGCTACTCCAATGGCGTCAGCCCCATGC
AGCGGAGCTCTAGCTTCAGCCTCCCTGCCAGGGCCAATGGGCTCTCTTCACCTTGCCACCAGTCGAGCCT
GCACCACCGCTTTGGGGGGCAACCCTGCCAAGGAGCACCAGGCTCAGCCCCCTGTGGACAGGCGGGTGAT
AGCTGGAGCCCAGACCCACACCCAGTGGGAGGGGGCCGGCTGGACCTGCAGCGGTCCCTCTCTTGCTCGC
ATGAGCAGTTTTCCTTCCCAGAATACTGTCCCCCCTCAGCCAACAGCACACCTGCCTCAACGCCAGAGCT
GGCAAGACGCTCCAGTGGGCTTGCCCGCAGCCGCTCTCAGCCCTGTGTCCTTAATGACAAGAAGATCGGT
GTGAAACGGCGGCGTCCGGATGAAGTGCAGGAGCAAAGACCTTCTCTAGACCTTGCCAAGATGGCACAGA
ACTGTCAGACCTTCAGCAGCCTCAGTTGCCTGAACATGGGGGTGGACGACCACAGCTCCCAGAGCCCCTT
TGCCCTCGTCAGCAGTACCCGATCCTGGACCGCATTGCTCTCAGCCTCCAGCCCAGGGGGCAGGACTCCT
GCTGGGACCCCAGTTCCTGAGCCTGTTCCCCACTCCTTTGATGACCAGTTCACCTGCCAGGAGGACCTAT
CCTGTGACGAGTCAGATGGCTGCAGTCTAGATGAGGATTGCTGCAGGAAGGGGGATCCGGCCACATCCTG
GCGGGACCGGGGAGCCTGCACGAACAGCCTCTGCTCTTTGGATGGCGAGCTGGACATTGAGCAGATAGAG
AACAACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_175268
Insert Size 1269 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175268.4, NP_780477.2
RefSeq Size 5148 bp
RefSeq ORF 1269 bp
Locus ID 77938
UniProt ID Q8BGR5
Cytogenetics 7 F3
Summary Acts as a regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) nuclear localization.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).
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Documents
Resources
"NM_175268" in other vectors (2)
SKU Description Size Price
MG206723 Fam53b (tGFP-tagged) - Mouse RIKEN cDNA A930008G19 gene (A930008G19Rik) 10 ug
$886.00
MR229829 Fam53b (myc-DDK-tagged) - Mouse family with sequence similarity 53, member B (Fam53b) 10 ug
$289.00 $457.00

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