Nasp (NM_001284229) Mouse Untagged Clone

CAT#: MC227616

Nasp (untagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nasp
• Synonyms: 5033430J04Rik; AI131596; AI317140; D4Ertd767e; Epcs32; Nasp-T
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227616 representing NM_001284229
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAGAGTCTACAGCCGCTGCTGCCATCGCCGCGGAGCTGGTTTCCGCTGACAAAATTGAAGATG
  CTCCTGCTCCTTCTACCTCGGCAGATAAAATGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTACT
  GGGATTAGGACAGAAACATCTGGTGATGGGTGACATTCCAGCAGCTGTTAATGCATTCCAGGAAGCAGCT
  AGTCTTTTAATGGAGAATGGAGTGTTGGGAAATGCCTTAGAAGGAGTGCATGTAGAAGAGGAAGAAGGAG
  AAAAAACAGAAGATGAATCTTTGGTAGAAAATAATGATAATGTAGATGAAACTGAAGGCTCAGAGGAAGA
  GGACAGAGAAAATGACAAGGCTGAAGAAACACCAAATGAATCAGTTCTTGAAAAAAAGTCTCTTCAAGAA
  AATGAAGAGGAGGAGATAGGGAATTTGGAACTTGCCTGGGATATGCTGGATTTAGCCAAGATCATTTTTA
  AGAGGCAAGAAACAAAAGAAGCCCAGCTTTATGCTGCACAAGCCCATCTTAAACTTGGAGAAGTTAGTGT
  TGAATCTGAGAATTATATCCAAGCTGTGGAGGAGTTTCAGGCTTGCCTAAGCCTGCAAGAACAATATTTG
  GAAGCTCACGATCGTCTCCTTGCAGAGACTCACTACCAATTAGGTTTGGCCTATGGTTACAACTCTCAGT
  ATGATGAAGCAGTAGCACAGTTTGGCAAATCTATCGATGTCATTGAAAAGAGAATGGCTGTACTCCATGA
  GCAGATGAAGGAGGCCGAAGGATCGTTTACTGAATATGAGAAAGAAATTGAGGAGCTGAAAGAACTGCTA
  CCTGAAATTCGAGAGAAGATAGAAGACGCAAAGGAATCTCAGCGGAGTGGGAATGTAGCCGAACTGGCAC
  TGAAAGCTACTCTGGTGGAGAGCTCTACTTCCGGTTTCACTCCTAGTGGAGCTGGTGCCTCAGTATCCAT
  GATTGCCAGTAGAAAACCAACAGATGGCGCTTCCTCATCAAATTGTGTGACTGATATTTCCCATCTTGTC
  AGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAGGATGATGCAAAGAAAGCTAAACAAGAGCCAG
  AGGTGAATGGAGGCAGTGGGGATGCTGTCTCCAGTGGAAAGGAAGTTTCAGAAAACATGGAGGCCGAGGC
  TGAGAATCAGGCTGAGAGCCAAACAGCAGAGGGGACCGTGGAGTCTGCAGCTACAATTAAAAGCACTGCA
  TGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284229
• Insert Size: 1266 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284229.1, NP_001271158.1
• RefSeq Size: 2037 bp
• RefSeq ORF: 1266 bp
• Locus ID: 50927
• UniProt ID: Q99MD9
• Cytogenetics: 4 53.24 cM
• Gene Summary: Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and linker H1 histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks two alternate in-frame exons in the coding region, compared to variant 2. It encodes isoform 3, which is shorter than isoform 2.