Nxf1 (NM_001276704) Mouse Untagged Clone

CAT#: MC227594

Nxf1 (untagged) - Mouse nuclear RNA export factor 1 (Nxf1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nxf1
• Synonyms: Mex67; Mvb1; Tap
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227594 representing NM_001276704
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGAAGGGAAGTCATACAACGAGCATGATGACCGTGTTAGTTTCCCTCAAAGAAGAAAGAAAG
  GCCGGGGGCCTTTCCGATGGAAGTGTGGTGAGGGGAATCGTAGGTCTGGAAGAGGTGGTTCTGGTGTACA
  GTCTTCCCGATTTGAGGAAGATGATGGCGATGTAGCAATGAATGATCCCCAGGATGGCCCCCGAGTAAGA
  TACAATCCCTATACCAACCGGCCTAATCGTCGGGGAGATGGTTGGCATGATCGAGATCGAATTCATATTA
  CTGTACGGAGAGACAGAGCTCCTGCAGAGAGAGGAGGGGCTGGCACCAGTCAAGATGGGACCACCAAGAA
  CTGGTTCAAGATTACAATTCCTTATGGTAGAAAGTATGACAAAACGTGGCTTCTGAGCATGATTCAGAGC
  AAATGCAGTGTCCCCTTCAATCCCATCGAGTTTCACTATGAAAATACACGGGCCCATTTTTTTGTGGAGG
  ATGCCACTACTGCTTCTGCATTAAAGGGTGTCAACCATAAGATCCAGGATCGAGAAAACAGAAGGATATC
  TATCATCATCAATGCTTCTGCTCCACCCTACACTGTACAGAATGAACTGAAGCCCGAACAAATAGAGCAG
  CTCAAGCTGATCATGAGCAAACGATATGATGGCAACCAACAAGCACTTGACCTCAAGGGGCTCCGCTCAG
  ACCCAGATTTGGTGGCCCAGAACATCGATGTTGTTCTAAACCGCAGAAGCTGTATGGCAGCCACCCTGCG
  GATCATTGAAGAGAACATTCCTGAGCTATTGTCTTTGAACTTGAGCAGCAACAGGCTATACAAGCTGGAT
  GATATGTCTAGCATTGTGCAGAAGGCGCCAAACCTAAAGACCCTAAATCTCTCCGGAAACGAATTGAAGA
  CTGAGCGGGAATTAGACAAGATAAAAGGGCTGAAGCTGGAAGAGCTGTGGCTTGACAGAAACCCCATGTG
  TGACAACTTCGGAGACCAGTCCAGCTATATCAGGTCAGTTGTAGCCTCTGTCTCCCCTCCTGGGGACATT
  CACCCCTGGGAGGCTGAGCTCATGCACCCTGACCAGTGCCCGTCTACAGGAGAACATGCAGCCCTGAGCT
  GGAACCACCTGTTCTTTGGGAGGAGGATCATGTATTCCTTTCTGCCTGTCTGTTGTCTCCTAGCTCTGTC
  CCAGATCTCTTCTTTCTTCTCACCTGACTTCTGTTCTCTAGTTCTGGCCTTTCTCCTTCTTTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276704
• Insert Size: 1257 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276704.1, NP_001263633.1
• RefSeq Size: 4035 bp
• RefSeq ORF: 1257 bp
• Locus ID: 53319
• Cytogenetics: 19 5.5 cM
• Gene Summary: Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate internal segment and transcription extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.