Pafah2 (NM_001285872) Mouse Untagged Clone

CAT#: MC227576

Pafah2 (untagged) - Mouse platelet-activating factor acetylhydrolase 2 (Pafah2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pafah2
• Synonyms: 2310074E22Rik; AI507170
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227576 representing NM_001285872
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGAAATAATAGAAGACCTCAGTTGGCCAGCAAATTCCCGAGCACCCGGCTTTGGTCCAGCTGGTG
  ATGAGATGGGGGCCGGCCAGTCTGTGTGCTTCCCACCCATCTCGGGACCCCACCACATTGGCTGCACGGA
  TGTGATGGAGGGTCACAGTCTTGAGGGGAGCCTCTTCCGACTCTTCTACCCTTGCCAAGCATCAGAGAAG
  TGCGAGCAGCCCCTGTGGATTCCCCGCTACGAGTACAGCATGGGTCTGGCCGATTACCTGCAGTATAACA
  AGCGCTGGGTGGGGTTGCTGTTCAACGTGGGTATCGGATCTTGTCGCTTGCCTGTTAGCTGGAACGGCCC
  CTTTAAGGCAAAGGAGTCTGGATACCCTTTGATCATCTTGTCCCATGGTCTAGGAGGGTTCAGGGCGTCA
  TACTCGGCCTTCTGCATGGAGCTGGCCTCTCGTGGCTTTGTGGTTGCTGCCGTAGAGCACAGGGACCAGT
  CAGCTGCGGCCACCTACTTCTGCAAGCCGACCTCCCAGGAGAGCTCTCCCGCTGAATCTCTGGAGGAGGA
  GTGGCTCCCCTTCCGTCGCATCAAGGAAGGGGAGAAGGAGTTCCACGTCAGGAATCCTCAGGTGCACCAG
  CGGGTGAAAGAGTGTGTGCGAGTGCTGCGGATCCTTCAGGACGCCTCTGCTGGGAAGACTGTCGTCAATG
  TCTTCCCAGGAGGACTGGATCTGATGACCTTGAAGGGCAGCATCGACAGGAACCGCGTAGCCGTGATGGG
  TCACTCCTTTGGAGGAGCTACGGCTGTTCTTGCTTTGACCCAGGAGGTCCAGTTTCGGTGTGCTATAGCC
  CTGGACGCTTGGATGTTCCCTCTGGAACGGGACTTTTACCCCAAAGCCCGGGGCCCTGTGTTCTTTATCA
  ACGTCGAGAAATTCCAGACGGTAGAGAGCGTCAACTTGATGAAGAAGATCTGTGCCCAGCACGAGCAGTC
  CAGGATCGTGACTGTCCTAGGCGCTGTTCATCGGAGTCAAACTGACTTTGCCTTTGTGACGGGCAACCTG
  ATTGCTAAATTCTTTTCCAGTAACTCTCGTGGTACCTTGGACCCTTACGAAAGTCAGGAGGTCATGGTGA
  GGGCCATCTTGGCCTTCCTGCAGAAGCACCTTGACCTGAAGGAAGACTACGATCAATGGAGCAGCTTCAT
  CGAAGGCGTCGGACCATCGCTCATCCAAGGGGCCCCGCACTATCTGTCTAGTCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285872
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285872.1, NP_001272801.1
• RefSeq Size: 3194 bp
• RefSeq ORF: 1248 bp
• Locus ID: 100163
• Cytogenetics: 4 D3
• Gene Summary: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).