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Crhr2 (NM_001288619) Mouse Untagged Clone

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SKU
MC227557
Crhr2 (untagged) - Mouse corticotropin releasing hormone receptor 2 (Crhr2), transcript variant 3
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ORF Plasmid:
Tag Free DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Crhr2
Synonyms CRF-R2; Crfr2; CRFR2alpha; CRFR2beta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227557 representing NM_001288619
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCGGCGCTGCTCCTCAGCCTGCTGGAGGCCAACTGCAGCCTGGCGCTGGCCGAAGAGCTGCTCC
TGGACGGCTGGGGAGTGCCCCCGGACCCCGAAGGTCCCTACACCTACTGCAACACGACCTTGGACCAGAT
CGGGACCTGCTGGCCACAGAGCGCACCCGGAGCCCTAGTAGAGAGACCGTGCCCCGAGTACTTCAATGGC
ATCAAGTACAACACGACCCGGAATGCCTACAGAGAGTGCCTGGAGAACGGGACCTGGGCCTCAAGGGTCA
ACTACTCACACTGCGAACCCATTTTGGATGACAAGCAGAGAAAGTATGACCTGCATTACCGAATCGCCCT
CATTGTCAACTACCTGGGTCACTGTGTTTCCGTGGTGGCCCTGGTGGCCGCTTTCCTGCTTTTCCTAGTG
CTGCGGAGTATCCGCTGCCTGAGGAATGTGATCCACTGGAACCTCATCACCACCTTCATTCTGAGAAACA
TCGCGTGGTTCCTGCTGCAACTCATCGACCACGAAGTGCACGAGGGCAATGAGGTCTGGTGCCGCTGCAT
CACCACCATCTTCAACTATTTTGTGGTCACCAACTTCTTCTGGATGTTTGTGGAGGGCTGCTACCTGCAC
ACGGCCATTGTCATGACGTACTCCACAGAGCACCTGCGCAAGTGGCTTTTCCTCTTCATTGGATGGTGCA
TTCCCTGCCCTATCATCATCGCCTGGGCAGTTGGCAAACTCTACTATGAGAATGAGCAGTGCTGGTTTGG
CAAGGAAGCTGGTGATTTGGTGGACTACATCTACCAGGGCCCCGTCATGCTTGTGCTGTTGATCAATTTT
GTATTTCTGTTTAACATCGTCAGGATCCTGATGACGAAGTTACGAGCATCCACCACGTCCGAGACAATCC
AATACAGGAAGGCAGTGAAGGCCACGCTGGTCCTCCTCCCCCTGTTGGGCATCACCTACATGCTCTTCTT
TGTCAATCCTGGCGAGGACGACCTGTCCCAGATTGTGTTCATCTACTTCAACTCTTTCCTGCAGTCCTTC
CAGGGTTTCTTTGTGTCCGTTTTCTACTGCTTCTTCAATGGAGAGGTGCGCGCGGCCCTGAGAAAGCGGT
GGCACCGCTGGCAGGACCACCACGCCCTCCGGGTGCCTGTGGCCCGGGCCATGTCCATCCCTACGTCGCC
CACCAGGATCAGCTTCCACAGCATCAAGCAGACAGCTGCTGTGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_001288619
Insert Size 1236 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001288619.1, NP_001275548.1
RefSeq Size 2607 bp
RefSeq ORF 1236 bp
Locus ID 12922
UniProt ID Q60748
Cytogenetics 6 27.33 cM
Summary G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1.
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Documents
Resources
"NM_001288619" in other vectors (1)
SKU Description Size Price
MR229768 Crhr2 (myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 2 (Crhr2), transcript variant 3 10 ug
$503.00

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