Tfdp1 (NM_001291765) Mouse Untagged Clone

CAT#: MC227551

Tfdp1 (untagged) - Mouse transcription factor Dp 1 (Tfdp1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tfdp1
• Synonyms: Dp1; Drtf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227551 representing NM_001291765
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAAGATGCCAGTCTAATTGAAGCCAACGGAGAACTAAAGGTCTTTATAGACCAGAATCTTAGTC
  CTGGGAAAGGTGTGGTATCTCTTGTAGCCGTCCACCCGTCCACAGTCAACACACTTGGGAAGCAGCTTTT
  GCCAAAAACCTTCGGACAGTCCAATGTCAATATCACACAGCAAGTGGTGATTGGCACGCCTCAGAGACCG
  GCAGCATCCAACACTATTGTGGTAGGAAGCCCACACACTCCCAACACGCATTTTGTGTCACAGAACCAGA
  CGTCTGACTCCTCACCTTGGTCTGCTGGGAAGCGGAACAGGAAGGGCGAGAAGAATGGCAAGGGCCTGCG
  GCATTTCTCCATGAAGGTGTGTGAGAAGGTGCAGAGGAAAGGAACCACCTCCTACAATGAGGTGGCTGAC
  GAGCTGGTGGCAGAGTTCAGCGCTGCCGACAACCACATTCTACCAAACGAATCAGCTTATGACCAGAAGA
  ACATCCGGCGGCGTGTCTACGATGCCTTAAATGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAA
  GGAGATCAAATGGATCGGCCTGCCCACCAACTCAGCTCAGGAGTGCCAGAACTTAGAGGTAGAGAGGCAG
  AGGAGGCTGGAGAGGATCAAACAGAAGCAGTCACAGCTCCAGGAGCTCATCCTGCAGCAAATTGCCTTCA
  AGAACTTGGTGCAGAGAAATCGCCAAGCTGAGCAGCAGGCCCGCAGGCCGCCTCCTCCCAACTCTGTCAT
  CCACTTGCCCTTCATCATTGTCAACACCAGCAGGAAGACAGTCATTGACTGCAGCATCTCCAATGACAAA
  TTTGAGTATCTGTTTAACTTTGACAACACGTTTGAGATCCACGATGACATTGAGGTGCTCAAGCGCATGG
  GCATGGCATGTGGGCTGGAGTCTGGCAACTGCTCTGCTGAAGACCTCAAGGTGGCCAGAAGTTTGGTACC
  AAAAGCTCTAGAACCATACGTGACAGAAATGGCTCAGGGATCCATTGGTGGAGTATTCGTCACGACAACA
  GGTTCTACATCCAATGGCACAAGGCTTTCTGCCAGTGATTTGAGCAATGGTGCAGATGGGATGCTGGCCA
  CGAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTCGAGACCCCTGTGTCCTACGTTGGGGAGGATGA
  TGACGACGATGATGACTTTAATGAGAACGACGAGGAGGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291765
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291765.1, NP_001278694.1
• RefSeq Size: 2523 bp
• RefSeq ORF: 1233 bp
• Locus ID: 21781
• UniProt ID: Q08639
• Cytogenetics: 8 A1.1
• Gene Summary: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).