Zfp322a (NM_001286156) Mouse Untagged Clone

CAT#: MC227548

Zfp322a (untagged) - Mouse zinc finger protein 322A (Zfp322a), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp322a
• Synonyms: Znf322; Znf322a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227548 representing NM_001286156
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAACCACTTAGGAGAGAACCATAGAAGATGTACACTTCAGAAGAGGAATGTAACCAGAGAACTCA
  AAAAAGGAAAACATACCATGTATGCCCTCAAAAGGGTGAAAAAGATTTATATTCGTGTACATGAGATTAC
  TCAAATAGATAATCAAACATATCAGTGCCTTGAACGTGAACAAAACTTTTGTGAAAACTTAGCACGAATG
  TGTGAGAGAACATATACCGAGGAGAAACCTTATAGATGTGATATGTGTGAGAAAACCTTCATCCAAAGTT
  CAGATCTTATTTCCCACCAGAGGATCCATAATTACGAGAAACCTTATAAGTGTAGCAAATGTGAGAAGAG
  CTTTTGGCACCACTTAGCCCTTTCAGGACACCAGAGAACACATGCAGGTAAAAAGTTTTATACCTGTGAC
  ATCTGTGGCAAGAATTTTGGTCAGAGCTCTGATCTGCTTGTCCACCAGCGAAGCCATACAGGTGAAAAAC
  CTTATCTGTGTAATGAGTGTGATAAATGCTTCAGTCGAAGTACAAATCTCATAAGGCACCGAAGAACTCA
  CACAGGTGAGAAACCGTTTAAGTGTCTGGAATGTGAAAAAGCTTTTAGTGGGAAATCAGATCTTATTAGC
  CACCAAAGGACTCATACCGGTGAAAGGCCCTACAAATGCAATAAATGTGAGAAAAGTTACCGACACCGGT
  CTGCCTTCATTGTGCATAAAAGAGTTCATACTGGGGAGAAGCCGTACAAGTGTGGTGCCTGTGAGAAGTG
  CTTTGGCCAGAAATCAGACCTTATTGTACACCAGAGAGTCCATACAGGAGAGAAACCATATAAATGCTTG
  GAGTGTATGAGAAGTTTTACTCGAAGTGCCAACCTAATTAGGCACCAGGCAACTCACACTCATACTTTCA
  AATGCCTTGAATATGAGAAAAGTTTCAACTGTAGCTCAGACTTTATTGTACATCAGAGAATTCATATGGA
  AGAAAAACCACATCAATGGTCTATGTGTGAGAGTGACTTCCTCCTAGGTATGGACTTCGTTGCCCAGCAG
  AAAATGAGGGCTCAAACAGAGGAGCTCCATTATAAATACAGTGTATGTGATAAAACCTTTCATCATAGCT
  CAGCCCTTCTTCAACATCAGACAGTGCACATTGATGATGAATACATCTGTAACATGAGTGAAAAAGGGCT
  TGATCTAAGTTCTCACGCATCGGAAACCTCACGTGTGTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286156
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286156.1, NP_001273085.1
• RefSeq Size: 5122 bp
• RefSeq ORF: 1233 bp
• Locus ID: 218100
• UniProt ID: Q8BZ89
• Cytogenetics: 13 A3.1
• Gene Summary: Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 3. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.