Cyp39a1 (NM_001285948) Mouse Untagged Clone

CAT#: MC227517

Cyp39a1 (untagged) - Mouse cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp39a1
• Synonyms: mCYP39A1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227517 representing NM_001285948
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATTATGGAGTTATTCTCACCAATAGCAATCGCCGTTCTGGGAAGCTGTGTTCTTTTCTTATTCT
  CTCGGTTGAAGAATTTGCTTGGACCCCCGTGCATCCAAGGCTGGATTCCCTGGATTGGAGCTGGACTTGA
  GTTTGGGAAAGCCCCTCTTGAATTTATAGAAAAAGCAAGAATCAAGTATGGACCGGTATTTACCATCTTT
  GCTATGGGAAACCGAATGACCTTTGTTAGTGAAGAAGAAGGAATCAATGTGCTTCTAAAATCTGAACATG
  TAGATTTTGAATCAGCAGTACAAAGTCCTGTCTATCACACAGCATGGATTCCAAAGAATGTCTTCTCTGC
  ACTGCATGAGCGACTGTATGCCTTGATGAAAGGGAAGATGGGAACTTTCAACACACATCACTTTACTGGG
  CCGCTAACTGAAGAACTTCATGAGCAACTGGAAGGTTTAGGCACTCATGGAACAATGGACCTGAATGACT
  TTGTAAGGTATCTACTTTATCCAGCCACACTCAATACCCTCTTTAAGAAAGGGTTGTTTCTCACAGACAA
  GAGGACAATCAAGGAATTCTACCAGCAGTTTAAAACCTATGATGAGGGCTTTGAGTATGGCTCCCAGCTG
  CCTGAATGGCTTCTGAGAAACTGGTCAAAATCCAAAAGATGGCTCCTGGCGCTGTTTGAGAAAAATATTG
  GCAACATAAAAGCTCACGGATCTGCAGGTCATTCTGGAACCCTCTTGCAGGCCATATTGGAAGTGGTGGA
  GACGGAAACACGTCAATACAGTCCAAATTATGGACTGGTCGTACTTTGGGCTGCTCTGGCCAATGCTCCT
  CCTATTGCATTCTGGACACTTGGATATATCCTGTCTCATCCCGATATCCACAGGACTGTTTTAGAAAGCA
  TATCTTCTGTGTTTGGCACTGCAGGTAAAGACAAGATTAAAGTGTCTGAAGATGACCTGAAGAAGCTTCT
  AATAATTAAGTGGTGCATTCTGGAATCTGTCCGCTTGAGAGCCCCTGGTGTCATTACTAGAAAGGTAGTG
  AAGCCAGTTAAAATTCTGAATCACACAGTTCCTTCTGGTGATCTGCTGATGTTGTCTCCATTCTGGCTAC
  ACAGAAATCCAAAATATTTTCCTGAACCTGAGTCATTCAAACCTGTGGTTTGCTCTCTTAGAGATTCAAC
  TATGCATTATCTTAGTGCTGTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285948
• Insert Size: 1215 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285948.1, NP_001272877.1
• RefSeq Size: 3017 bp
• RefSeq ORF: 1215 bp
• Locus ID: 56050
• Cytogenetics: 17 B3
• Gene Summary: Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an exon in the 3' coding region resulting in a frameshift compared to variant 1. The encoded protein (isoform 3) has a distinct C-terminus and is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.