Ncf1 (NM_001286037) Mouse Untagged Clone

CAT#: MC227516

Ncf1 (untagged) - Mouse neutrophil cytosolic factor 1 (Ncf1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ncf1
• Synonyms: Ncf-1; NCF-47K; NOXO2; p47-phox; p47; p47phox
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227516 representing NM_001286037
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACACCTTCATTCGCCATATTGCCCTGCTGGGCTTCGAGAAGCGCTTCATCCCCAGCCAGCACT
  ATGTGTACATGTTCCTGGTTAAGTGGCAGGACCTGTCGGAGAAGGTGGTCTACAGAAAATTCACCGAGAT
  CTACGAGTTCCATAAAATGCTGAAGGAGATGTTCCCCATTGAGGCCGGCGAGATCCACACAGAGAACAGA
  GTCATCCCACACCTCCCGGCACCCAGGTGGTTTGATGGGCAACGAGCCGCTGAGAGTCGCCAGGGCACTC
  TCACTGAATACTTCAACGGCCTCATGGGACTGCCCGTGAAGATCTCCCGCTGCCCACACCTGCTGGACTT
  CTTCAAAGTGCGGCCTGATGACCTGAAACTGCCCACTGACAGCCAGGCGAAGAAGCCTGAGACATACCTG
  GTGCCCAAAGATGGCAAGAATAACGTAGCTGACATCACAGGCCCCATCATCCTTCAGACCTATCGGGCCA
  TTGCTGACTACGAGAAGAGTTCGGGAACAGAGATGACCGTGGCAACCGGAGACGTGGTGGACGTTGTGGA
  GAAGAGCGAGAGCGGCTGGTGGTTTTGCCAGATGAAGACAAAGCGAGGTTGGGTCCCTGCATCCTATCTG
  GAGCCCCTTGACAGTCCCGACGAGGCGGAGGATCCGGATCCCAACTACGCAGGTGAACCGTATGTAACCA
  TCAAAGCGTACGCTGCTGTTGAAGAGGACGAGATGTCCCTGTCTGAGGGTGAAGCCATTGAGGTCATTCA
  TAAGCTCCTGGATGGCTGGTGGGTGGTCAGGAAAGGGGATATCACCGGCTATTTCCCATCCATGTATCTG
  CAGAAGGCTGGGGAGGAGATAACCCAGGCCCAGCGACAGATCAGAGGCCGCGGGGCACCACCTCGAAGGT
  CGACCATCCGCAACGCACAGAGCATCCACCAGCGTTCTCGGAAGCGCCTTAGCCAGGACACCTATCGCCG
  CAACAGCGTCCGATTCCTGCAGCAGCGCAGACGCCCGGGGCGACCCGGGCCGCTGAGCACGGATGGCACA
  AAGGGTGAGCGCCAGGGCGGGCCGGGGCCTGGGCTGGAGGGCAGAGACAATCCATCGACTCCGCGCGTCA
  AACCACAGCCCGCGGTGCCTCCGCGACCCAGCTCGGACCTCATCCTGCACCGCTGCACAGAGAGCACCAA
  ACGGAAGCTGACGTCCGCTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286037
• Insert Size: 1215 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286037.1, NP_001272966.1
• RefSeq Size: 2764 bp
• RefSeq ORF: 1215 bp
• Locus ID: 17969
• UniProt ID: Q09014
• Cytogenetics: 5 74.47 cM
• Gene Summary: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).