St6gal1 (NM_001252506) Mouse Untagged Clone

CAT#: MC227511

St6gal1 (untagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 1 (St6gal1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: St6gal1
• Synonyms: AW742324; Si; Siat1; St6G; St6gal; St6Gal-I; St6galI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227511 representing NM_001252506
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCATACCAACTTGAAGAGAAAGTTCAGCTGCTTTGTCCTGGTCTTTCTCCTGTTTGCCATCATCT
  GCGTGTGGAAGAAAGGGAGCGACTATGAGGCTCTTACATTGCAAGCCAAGGTATTCCAGATGCCGAAGAG
  CCAGGAGAAAGTGGCCGTGGGGCCTGCTCCCCAGGCTGTGTTCTCAAACAGCAAACAAGACCCTAAGGAA
  GGCGTTCAGATCCTCAGTTACCCCAGGGTCACAGCCAAGGTCAAGCCACAGCCCTCCTTGCAGGTGTGGG
  ACAAGGACTCCACATACTCAAAACTTAACCCCAGGCTGCTGAAGATCTGGAGGAACTATCTGAACATGAA
  TAAATATAAAGTGTCCTACAAGGGGCCGGGACCAGGAGTCAAGTTCAGCGTAGAGGCGCTGCGCTGCCAC
  CTTCGAGACCACGTGAATGTGTCTATGATAGAGGCCACAGATTTTCCCTTCAACACCACTGAATGGGAGG
  GTTACCTGCCCAAGGAGAACTTCAGAACCAAGGCTGGGCCTTGGCATAAGTGTGCCGTCGTGTCTTCTGC
  AGGATCTCTGAAGAACTCCCAGCTGGGTCGAGAGATTGATAATCATGATGCGGTCCTGAGGTTTAATGGG
  GCACCTACAGACAACTTCCAACAGGATGTGGGCACAAAAACTACCATCCGCCTAGTGAACTCTCAGTTAG
  TCACCACAGAAAAGCGCTTCCTGAAGGACAGTTTGTACACCGAAGGAATCCTGATTCTGTGGGACCCATC
  TGTGTATCATGCAGACATTCCGCAGTGGTATCAGAAGCCAGACTACAACTTCTTCGAAACCTATAAGAGT
  TACCGAAGGCTTCACCCCAGCCAGCCTTTTTACATCCTCAAGCCCCAGATGCCATGGGAACTATGGGACA
  TCATTCAGGAAATCTCTCCAGATCTGATTCAGCCGAATCCCCCATCCTCCGGCATGCTGGGTATCATCAT
  TATGATGACGCTGTGTGACCAAGTTGATATTTACGAGTTCCTCCCATCCAAGCGCAAGACAGATGTGTGC
  TACTATCACCAGAAGTTCTTTGACAGCGCCTGCACGATGGGTGCCTACCATCCGCTCCTCTTCGAGAAGA
  ATATGGTGAAGCATCTCAATGAGGGAACAGATGAAGACATTTATTTGTTTGGGAAAGCTACCCTGTCTGG
  CTTCCGGAACAATCGCTGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252506
• Insert Size: 1212 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252506.1, NP_001239435.1
• RefSeq Size: 4044 bp
• RefSeq ORF: 1212 bp
• Locus ID: 20440
• UniProt ID: Q64685
• Cytogenetics: 16 14.03 cM
• Gene Summary: This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on chromosome 15. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.