St6gal1 (NM_001252505) Mouse Untagged Clone

SKU
MC227510
St6gal1 (untagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 1 (St6gal1), transcript variant 2
  $503.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol St6gal1
Synonyms AW742324; Si; Siat1; St6G; St6gal; St6Gal-I; St6galI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227510 representing NM_001252505
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCATACCAACTTGAAGAGAAAGTTCAGCTGCTTTGTCCTGGTCTTTCTCCTGTTTGCCATCATCT
GCGTGTGGAAGAAAGGGAGCGACTATGAGGCTCTTACATTGCAAGCCAAGGTATTCCAGATGCCGAAGAG
CCAGGAGAAAGTGGCCGTGGGGCCTGCTCCCCAGGCTGTGTTCTCAAACAGCAAACAAGACCCTAAGGAA
GGCGTTCAGATCCTCAGTTACCCCAGGGTCACAGCCAAGGTCAAGCCACAGCCCTCCTTGCAGGTGTGGG
ACAAGGACTCCACATACTCAAAACTTAACCCCAGGCTGCTGAAGATCTGGAGGAACTATCTGAACATGAA
TAAATATAAAGTGTCCTACAAGGGGCCGGGACCAGGAGTCAAGTTCAGCGTAGAGGCGCTGCGCTGCCAC
CTTCGAGACCACGTGAATGTGTCTATGATAGAGGCCACAGATTTTCCCTTCAACACCACTGAATGGGAGG
GTTACCTGCCCAAGGAGAACTTCAGAACCAAGGCTGGGCCTTGGCATAAGTGTGCCGTCGTGTCTTCTGC
AGGATCTCTGAAGAACTCCCAGCTGGGTCGAGAGATTGATAATCATGATGCGGTCCTGAGGTTTAATGGG
GCACCTACAGACAACTTCCAACAGGATGTGGGCACAAAAACTACCATCCGCCTAGTGAACTCTCAGTTAG
TCACCACAGAAAAGCGCTTCCTGAAGGACAGTTTGTACACCGAAGGAATCCTGATTCTGTGGGACCCATC
TGTGTATCATGCAGACATTCCGCAGTGGTATCAGAAGCCAGACTACAACTTCTTCGAAACCTATAAGAGT
TACCGAAGGCTTCACCCCAGCCAGCCTTTTTACATCCTCAAGCCCCAGATGCCATGGGAACTATGGGACA
TCATTCAGGAAATCTCTCCAGATCTGATTCAGCCGAATCCCCCATCCTCCGGCATGCTGGGTATCATCAT
TATGATGACGCTGTGTGACCAAGTTGATATTTACGAGTTCCTCCCATCCAAGCGCAAGACAGATGTGTGC
TACTATCACCAGAAGTTCTTTGACAGCGCCTGCACGATGGGTGCCTACCATCCGCTCCTCTTCGAGAAGA
ATATGGTGAAGCATCTCAATGAGGGAACAGATGAAGACATTTATTTGTTTGGGAAAGCTACCCTGTCTGG
CTTCCGGAACAATCGCTGTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001252505
Insert Size 1212 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001252505.1, NP_001239434.1
RefSeq Size 4177 bp
RefSeq ORF 1212 bp
Locus ID 20440
UniProt ID Q64685
Cytogenetics 16 14.03 cM
Summary This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on chromosome 15. provided by RefSeq, Nov 2011
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.
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Documents
Resources
"NM_001252505" in other vectors (1)
SKU Description Size Price
MR229721 St6gal1 (myc-DDK-tagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 1 (St6gal1), transcript variant 2 10 ug
$289.00 $457.00

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