Steap2 (NM_001285471) Mouse Untagged Clone

CAT#: MC227507

Steap2 (untagged) - Mouse six transmembrane epithelial antigen of prostate 2 (Steap2), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Steap2
• Synonyms: 4921538B17Rik; AI930049; AW045895; IPCA-1; IPCA1; PCANAP1; STAMP1; STMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227507 representing NM_001285471
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCCATCTCTATGATGGGAAGCCCTAAGAGCCTGGAGACGTTTTTGCCTAATGGCATAAATGGTA
  TCAAAGACGCAAGGCAGGTCACCGTGGGGGTGATAGGAAGTGGGGATTTTGCCAAGTCTCTGACCATTCG
  GCTTATTAGATGTGGCTACCACGTGGTCATAGGAAGCAGAAATCCCAAGTTTGCATCAGAATTTTTTCCT
  CACGTGGTAGACGTCACCCACCATGAAGATGCTTTAACAAAAACAAATATAATATTCGTGGCTATCCATA
  GAGAACATTACACCTCCTTGTGGGACCTGAGACATCTGCTTGTGGGCAAAATCCTCATTGATGTGAGCAA
  CAACATGAGAGTAAACCAGTACCCAGAATCCAATGCAGAGTACCTGGCTTCATTATTCCCGGACTCCTTG
  ATTGTCAAAGGATTTAATGTGATCTCAGCTTGGGCACTTCAGCTAGGTCCCAAGGATGCCAGCCGCCAGG
  TTTATATATGCAGCAACAATATCCAAGCTCGACAGCAGGTTATCGAGCTCGCCCGCCAGCTGAATTTTAT
  TCCTGTTGACTTGGGATCTTTGTCGTCAGCCAAGGAGATTGAAAACTTACCTCTGCGACTGTTTACTCTC
  TGGAGGGGGCCAGTGGTAGTAGCCATAAGCTTGGCCACATTTTTCTTTCTTTATTCCTTTGTCAGAGATG
  TGATACATCCATATGCCAGAAACCAGCAGAGTGACTTTTACAAGATTCCCATTGAGATTGTGAACAAAAC
  CTTGCCGATCGTCGCCATCACCCTGCTGTCTCTGGTGTACCTGGCTGGCCTCCTGGCAGCTGCGTATCAG
  CTTTATTATGGCACTAAGTACCGCCGATTTCCCCCGTGGCTGGATACTTGGCTGCAGTGCAGGAAACAGC
  TGGGATTGCTGAGCTTCTTCTTTGCAGTTGTTCACGTAGCCTACAGCCTCTGCTTACCAATGAGGAGGTC
  GGAAAGATACCTGTTCCTCAACATGGCTTATCAGCAGGTTCATGCCAATATTGAGAACGCGTGGAACGAG
  GAGGAGGTCTGGAGGATTGAGATGTACATTTCCTTTGGCATCATGAGCCTGGGCTTGTTGTCCCTGCTGG
  CGGTCACTTCCATCCCATCAGTGAGCAACGCTTTGAACTGGAGAGAGTTCAGTTTCATCCAGTGGCCCAG
  AGCTGCCCTGTCCTCCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001285471
• Insert Size: 1209 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285471.1, NP_001272400.1
• RefSeq Size: 4624 bp
• RefSeq ORF: 1209 bp
• Locus ID: 74051
• Cytogenetics: 5 A1
• Gene Summary: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) differs in the 5' UTR and contains an alternate splice site in the 3' terminal exon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.