Kcnj15 (NM_001271687) Mouse Untagged Clone

CAT#: MC227501

Kcnj15 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 15 (Kcnj15), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnj15
• Synonyms: 4930414N08Rik; AI182284; AI267127; IRKK; Kir4.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227501 representing NM_001271687
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGCCAGGTGGGAGAAGGGGAGCGAGGACGCGCCACTTACCCTGCAGAAGATTCCTGACCTACAGA
  GTGGTCCAAAGATGGATGCCATTCACCTTGGCATGTCCAGTGCCCCACTGGTGAAGCATACCAACGGGGT
  TGGACTCAAGGCCCACAGACCCCGAGTCATGTCAAAGAGTGGGCACAGTAATGTGAGAATCGATAAGGTA
  GACGGAATCTATTTACTCTACCTCCAGGACTTGTGGACAACCGTCATCGACATGAAGTGGCGATACAAGC
  TCACCCTATTTGCTGCCACCTTTGTGATGACCTGGTTTCTGTTTGGAGTGGTCTACTATGCCATAGCCTT
  TATTCATGGTGACTTACAACTTGGGGAATCTAATTCCAACCACACACCCTGCATTATGAAAGTGGACTCT
  CTCACAGGAGCATTCCTCTTTTCCTTGGAATCTCAGACAACCATTGGCTACGGGGTCCGTTCCATCACAG
  AGGAGTGTCCCCATGCTATCTTCCTCTTAGTCGCCCAACTGGTCATCACCACATTGATTGAGATCTTCAT
  TACGGGGACCTTTCTGGCTAAAATTGCAAGACCCAAAAAGCGAGCCGAGACCATTAAGTTCAGCCACTGT
  GCTGTCATCAGCAAGCAGAATGGAAAGCTATGCCTGGTCATCCAGGTGGCCAACATGAGGAAGAGTCTCC
  TGATTCAGTGCCAGCTCTCTGGAAAACTCCTGCAGACACACGTCACCAAAGAGGGAGAACGCATTCTCCT
  CAACCAGGCCACTGTCAAATTCCACGTGGACTCCTCTTCCGAGAGTCCCTTCCTCATCCTGCCCATGACC
  TTCTACCACGTGTTGGATGAGACAAGCCCCCTGCGGGACCTCACACCCCAAAACCTAAAGGAGAAGGAGT
  TTGAGCTGGTGGTACTTCTCAACGCCACGGTGGAGTCTACCAGCGCCGTCTGCCAGAGCCGAACGTCTTA
  CATCCCGGAGGAGATCTACTGGGGCTTTGAGTTTGTGCCTGTGGTTTCTCTCTCCAAAAATGGAAAGTAT
  GTGGCTGATTTCAGTCAATTTGAGCAGATCAGGAAGAGCCCGGATTGTACCTTCTACTGTGCCGATTCTG
  AGAAGCAGAAGCTTGAAGAACAGTACAGGCAAGAGGACCAGAGGGAGCGGGAGCTGAGGAGCCTCCTGCT
  ACAGCAGAGCAATGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271687
• Insert Size: 1209 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271687.1, NP_001258616.1
• RefSeq Size: 5210 bp
• RefSeq ORF: 1209 bp
• Locus ID: 16516
• Cytogenetics: 16 55.86 cM
• Gene Summary: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4, 6, 8, and 10 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.