Crtam (NM_001281954) Mouse Untagged Clone

CAT#: MC227465

Crtam (untagged) - Mouse cytotoxic and regulatory T cell molecule (Crtam), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Crtam
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227465 representing NM_001281954
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTGGGGAGCCCTCAGTTTGCTGTTCTGGGTGCCGGTGCAAGCAGCCTTTCTGAAAATGGAGACCG
  TCACGGTAGAGGAAGGCCAAACGCTCACTCTAACGTGTGTTACTTCTCAGACAAAAAACGTCTCTCTCCA
  GTGGCTGGCTCCCTCTGGGTTCACCATTTTTTTAAACCAGCATCCTGCTTTAAAAAGCTCCAAATACCAG
  CTTCTTCACCATTCAGCTACACAACTCTCCATTAGTGTGTCCAATGTAACTCTCCGAGAGGAAGGTGTGT
  ATACATGCTTGCACTACGGGAGTTCTGTGAAGACGAAGCAAGTGAGAGTGACCGTGTTAGTGACTCCTTT
  CCAGCCAACCGTGGAAGCTTTGGTTCTCAGAAGGCAGAATGGAGAGAAATCGGTTGTGCTGAAATGTTCC
  ACAGAGAGAAGCAAGCCCCCGCCACAAATCACCTGGCTGCTGGGGGAAGGTCTGGAGATCTATGGTGAAC
  TCAACCATGAATTTGAAGCTGATGGGAAAATATGTAACACCAGCAGCATGCTCATCGCCCGCGCATACGG
  CAAAAACTCAACCGTGCACTGCATTATCCAGCACGAGGGCTTGCACGGGAGAAAGCTGGTTGCCCCCTTC
  CAGTTTGAAGATTTGGTTGCAGATCAAGAAACTTCAGATCAAGAAACTTCAGATGCCCCTGAGCAGAGCT
  CTCTCTCCTCCCAAGCCCTCCAGCAGCCCACAAGTACAGTCTCAATGATGGAAAATTCCAGTATACCAGA
  GACTGACAAGGAAGAGAAAGAACATGCCACTCAAGACCCTGGCTTGTCCACTGAAGCCAGTGCTCAGCAT
  ACAGGACTGGCCCGGAGGAAGAGTGGTATCCTGCTGCTCACACTGGTGTCCTTCCTCATTTTCATCCTTT
  TCATCATCGTTCAGCTCTTCATCATGAAGCTGCGTAAAGCACACGTGGTATGGAAGAAGGAAAGTGAAAT
  TTCAGAGCAAGCTCTAGAAAGTTACAGATCAAGATCCAACAACGAGGAGACATCTTCCCAGGAGAATAGC
  AGCCAGGCTCCCCAGTCTAAGCGTTGCATGAACTACATCACGCGGTTATACTCGGGAGCCAAAACAAAGA
  AGAGTGCCCAGCACTGGAAGCTCGGAGGCAAGCACAGCCGTGTCCCGGAGAGTATTGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281954
• Insert Size: 1182 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281954.1, NP_001268883.1
• RefSeq Size: 1939 bp
• RefSeq ORF: 1182 bp
• Locus ID: 54698
• UniProt ID: Q149L7
• Cytogenetics: 9 21.79 cM
• Gene Summary: Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.