Ager (NM_001271422) Mouse Untagged Clone

CAT#: MC227461

Ager (untagged) - Mouse advanced glycosylation end product-specific receptor (Ager), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ager
• Synonyms: RAGE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227461 representing NM_001271422
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCGGGGACAGCAGCTAGAGCCTGGGTGCTGGTTCTTGCTCTATGGGGAGCTGTAGCTGGTGGTC
  AGAACATCACAGCCCGGATTGGAGAGCCACTTGTGCTAAGCTGTAAGGGGGCCCCTAAGAAGCCGCCCCA
  GCAGCTAGAATGGAAACTGAACACAGGAAGAACTGAAGCTTGGAAGGTCCTCTCTCCCCAGGGAGGCCCC
  TGGGACAGCGTGGCTCGAATCCTCCCCAATGGTTCCCTCCTCCTTCCAGCCACTGGAATTGTCGATGAGG
  GGACTTTCCGGTGTCGGGCAACTAACAGGCGAGGGAAGGAGGTCAAGTCCAACTACCGAGTCCGAGTCTA
  CCAGATTCCTGGGAAGCCAGAAATTGTGGATCCTGCCTCTGAACTCACAGCCAGTGTCCCTAATAAGGTG
  GGGACATGTGTGTCTGAGGGAAGCTACCCTGCAGGGACCCTTAGCTGGCACTTAGATGGGAAACTTCTGA
  TTCCCGATGGCAAAGAAACACTCGTGAAGGAAGAGACCAGGAGACACCCTGAGACGGGACTCTTTACACT
  GCGGTCAGAGCTGACAGTGATCCCCACCCAAGGAGGAACCCATCCTACCTTCTCCTGCAGTTTCAGCCTG
  GGCCTTCCCCGGCGCAGACCCCTGAACACAGCCCCCATCCAACTCCGAGTCAGGGAGCCTGGGCCTCCAG
  AGGGCATTCAGCTGTTGGTTGAGCCTGAAGGTGGAATAGTCGCTCCTGGTGGGACTGTGACCTTGACCTG
  TGCCATCTCTGCCCAGCCCCCTCCTCAGGTCCACTGGATAAAGGATGGTGCACCCTTGCCCCTGGCTCCC
  AGCCCTGTGCTGCTCCTCCCTGAGGTGGGGCACGAGGATGAGGGCACCTATAGCTGCGTGGCCACCCACC
  CTAGCCACGGACCTCAGGAAAGCCCTCCTGTCAGCATCAGGGTCACAGGCTCTGTGGGTGAGTCTGGGCT
  GGGTACGCTAGCCCTGGCCTTGGGGATCCTGGGAGGCCTGGGAGTAGTAGCCCTGCTCGTCGGGGCTATC
  CTGTGGCGAAAACGACAACCCAGGCGTGAGGAGAGGAAGGCCCCGGAAAGCCAGGAGGATGAGGAGGAAC
  GTGCAGAGCTGAATCAGTCAGAGGAAGCGGAGATGCCAGAGAATGGTGCCGGGGGACCGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271422
• Insert Size: 1182 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271422.1, NP_001258351.1
• RefSeq Size: 1360 bp
• RefSeq ORF: 1182 bp
• Locus ID: 11596
• UniProt ID: Q62151
• Cytogenetics: 17 B1
• Gene Summary: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Can also bind oligonucleotides. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. RAGE-dependent signaling in microglia contributes to neuroinflammation, amyloid accumulation, and impaired learning/memory in a mouse model of Alzheimer disease.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.