Sapcd2 (NM_001290407) Mouse Untagged Clone

CAT#: MC227454

Sapcd2 (untagged) - Mouse suppressor APC domain containing 2 (Sapcd2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sapcd2
• Synonyms: 2010317E24Rik; 6030458L21Rik; AL033337; ang
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227454 representing NM_001290407
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGGCAGCGATGGCCGAGCGTGGCCGCCTATCGCACGCTGCGCCCGCTCCTAGTACAGAGGGGC
  TACCGCGAGCCTTTCTCCAGAGCCTGCGCACCCTCTTCGACATCCTGGACGACCGGCAACGCGGCTACGT
  GCACCTGCGGGAGATCGAGTCCCGCTGGCAAGGTGCAGACGCACGCGAGCTGCCGTGCGGCGTGCTCGAG
  GGCCTGCGCCAGGTGGCCCCGGCCAATGGCTACCTGACCTTCGAGCGTTTCGTAGCGGGCTTGCGCACCT
  CGCTGCTGAAAGCCGACGGCGGCCAGCGGGATCAGGCACGCGTCGCGGCCCGGCCTGGGGACCAGTCGTC
  CCTGCAGCAGCGCCTGATGTTCGCACCGGCCGATGAGCCGCGGACTGTCCTGGAGAGAAAGCCTCTGCCT
  CTGAGTGCGTGCCCTGCTTCTGGTGGCCCCAGTGGCACCTCCCGGAACCCTGAGCTGTTATGTGTCCCGG
  TGGAGGCGGCGTCCTGCCCCACAGAGACCGAGCGGCCCCTGAGCAAGGCGCTGGAGCAAATCCCCAGCGC
  GGATTTGGGTGCAGCAGCCTGTAAGACCCTGGGCAAGGGCACAGGTGAAGCCCGGCAGGCTCCACGGGCC
  CGAGGCGAGCGTCGAAGGCATACCATCACCAATGGTGTGGACTGCAGCCTGTTGAAGCAGATGAAGGAGT
  TAGACCAGGAACAGGAGGTGCTTCTGCAGGGCCTGGAGATGATGGCACGGGGCCGAGACTGGTACCAGCA
  GCAGCTGCAGCGTGTGCAGGAACGTCAGCGCCGCCTGAGCCAGAGCAGAGCTGCTGCAGACTTTGGGGCT
  GAAGGAAGCCCCCGCCCTCTGGGGAGGTTGCTGCCCAAAGTACAGGAGGTGGCCCGGTGCCTGGGGGAGC
  TGTTGACTGCAGCCTGCTCTGGCAGAGCTCTGCCTTCGTCCTCTTTAGGGCCCCTGGGCCCTCCCTCACC
  CTCAACTCCAGTCTGGCAGCAACAGACCATCCTCATGCTGAAAGAACAGAACCGGCTTCTCACTCAGGAG
  GTGACTGATAAGAGTGAGCGCATCACGCAGCTGGAGCAGGAGAAGTCTGCACTCATCAAGCAGCTGTTTG
  AGGCCCGAGCGCTCAGCCAGCAGGATTCGGGACCTCTGGACTCCACTTTCATCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001290407
• Insert Size: 1176 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290407.1, NP_001277336.1
• RefSeq Size: 3305 bp
• RefSeq ORF: 1176 bp
• Locus ID: 72080
• UniProt ID: Q9D818
• Cytogenetics: 2 A3
• Gene Summary: Plays a role in planar mitotic spindle orientation in retinal progenitor cells (RPCs) and promotes the production of symmetric terminal divisions (PubMed:26766442). Negatively regulates the mitotic apical cortex localization of GPSM2 (By similarity). Involved also in positive regulation of cell proliferation and tumor cell growth (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.