Asmt (NM_001199212) Mouse Untagged Clone

SKU
MC227437
Asmt (untagged) - Mouse acetylserotonin O-methyltransferase (Asmt)
  $503.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Asmt
Synonyms Hio; Hiomt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_001199212
Insert Size 1164 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001199212.1, NP_001186141.1
RefSeq Size 1273 bp
RefSeq ORF 1164 bp
Locus ID 107626
UniProt ID D3KU66
Cytogenetics X
Summary This gene belongs to the methyltransferase superfamily and is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin and is abundant in the pineal gland. Two amino acid substitutions (R78G and R242C) are present in the encoded protein derived from the reference strain, C57BL/6J, and this protein shows low enzyme activity relative to the protein derived from other strains. provided by RefSeq, May 2015
Transcript Variant: This variant (1) is based on a transcript from the C3H/HeJ strain and encodes an isoform (1) with high enzyme activity.
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Documents
Resources
"NM_001199212" in other vectors (1)
SKU Description Size Price
MR229648 Asmt (myc-DDK-tagged) - Mouse acetylserotonin O-methyltransferase (Asmt) 10 ug
$503.00

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