Nap1l4 (NM_001285489) Mouse Untagged Clone

CAT#: MC227435

Nap1l4 (untagged) - Mouse nucleosome assembly protein 1-like 4 (Nap1l4), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nap1l4
• Synonyms: 2810410H14Rik; AI316776; D7Wsu30e; Nap2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227435 representing NM_001285489
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAAACAGTCTTTCAGATGGAGGCCCTGCAGATTCTGTGGAAGCTGCTAAAAATGCCAGTAATA
  CAGAAAAACTCACAGACCAGGTGATGCAGAACCCACAAGTGCTGGCAGCTTTGCAGGAACGTCTTGACAA
  CGTCTCTCACACTCCTTCTAGTTACATAGAAACTTTACCTAAAGCTGTCAAAAGAAGAATTAATGCCCTG
  AAGCAGCTCCAGGTGAGGTGTGCGCACATAGAAGCTAAGTTCTACGAGGAAGTTCATGACCTGGAGAGGA
  AGTATGCAGCCTTGTACCAGCCTCTATTTGACAAGAGAAGAGAATTCATCACTGGTGACGTGGAGCCCAC
  AGATGCAGAGTCAGCGTGGCACAGTGAGAATGAGGAGGAGGACAAGTTGGCTGGAGATATGAAGAATAAA
  GTAGTCATAGCTGAGAAAGAAGCAGCAACAGTGGAAGAGCTGAACCCCAAAGGCATCCCTGAGTTCTGGT
  TCACCATCTTCAGAAATGTGGACATGCTTAGTGAGCTGGTGCAGGAATATGATGAACCGATCTTGAAACA
  TCTGCAGGATATTAAAGTGAAGTTTTCAGACCCTGGACAACCTATGTCTTTTGTGCTAGAGTTTCACTTT
  GAACCCAACGACTACTTCACCAATCCTGTCCTGACGAAAACGTACAAGATGAAATCAGAGCCAGACAAGG
  CCGACCCGTTTTCTTTTGAAGGTCCTGAAATTGTGGACTGTGATGGGTGTACAATAGACTGGAAGAAAGG
  AAAAAATGTCACAGTCAAAACCATCAAGAAAAAGCAGAAGCACAAGGGCCGGGGCACCGTACGAACCATC
  ACCAAGCAAGTGCCCAATGAGTCCTTCTTCAACTTCTTCAGCCCCCTGAAAGCCTCTGGAGATGGAGAAT
  CTCTGGATGAAGATTCCGAGTTCACCTTAGCCTCTGACTTTGAAATTGGGCACTTTTTTCGTGAGCGCAT
  CGTGCCACGGGCTGTCCTCTACTTCACTGGGGAGGCCATAGAGGATGATGACAATTTTGAAGAAGGCGAG
  GAAGGTGAAGAGGAGGAATTGGAAGGTGATGAAGAAGGAGAAGATGAGGATGATGCTGATGTTAACCCCA
  AGAAAGAACCCAGCCAGCCAGCGGAGTGCAAGCAGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285489
• Insert Size: 1161 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285489.1, NP_001272418.1
• RefSeq Size: 2299 bp
• RefSeq ORF: 1161 bp
• Locus ID: 17955
• UniProt ID: Q78ZA7
• Cytogenetics: 7 88.24 cM
• Gene Summary: Acts as histone chaperone in nucleosome assembly (PubMed:28366643). In condensing spermatids, mediates the loading of the heterodimer composed of histones H2AFB1 and HIST1H2BA/TH2B onto the nucleosomes, thereby promoting the replacement of histones to protamine in male germ cells (PubMed:28366643).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1 and 3 both encode the same isoform (a).