Ntn5 (NM_001289693) Mouse Untagged Clone

CAT#: MC227418

Ntn5 (untagged) - Mouse netrin 5 (Ntn5), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ntn5
• Synonyms: Gm484
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227418 representing NM_001289693
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGACTACAGGACACTCTTTTCTTCTCCAGGCCCCAAGACCAGGATAGTGGCCAGCTACCTCCGTG
  TGGAGTTCGGGGGAAAAGCAGGGCTGGTGACCACTGGAGTAAGAGGCCGCTGCCAGTGCCACGGCCACGC
  TGCCCGCTGTGCCACTCGGGCCCAGCCCCCACGCTGCCGCTGCCGCCACCACACCACTGGCCCAGGGTGT
  GAGAGCTGTCGCCCATCCCATCGAGACTGGCCCTGGCGACCTGCCACACCCCAGCACCCTCACCCGTGCT
  TGCCCTGTTCCTGCAATCAGCATGCCCGCCGCTGCCGATTCAACTCCGAGCTATTCAGGTTGTCAGGTGG
  CCGTAGTGGGGGTGTGTGTGAGCGGTGCCGCCACCACACAGCTGGGCGGCACTGTCACTACTGCCAGCCA
  GGGTTCTGGAGGGACCCGAGCCAGCCCATCACTAGTCACAAGGCATGCAGGGCCTGCCAGTGCCACCCAA
  TTGGAGCAACGGGAGGGATGTGCAACCAGACCAGTGGCCAGTGCTCCTGCAAGTTAGGGGTCACAGGCCT
  GACATGCAATCGCTGTGGTCCTGGCTACCAGCAGAGCCGCTCACCCAGGATGCCCTGCCAACGAATTCCA
  GAGGCAACAACTACCCCTGCTACTACCCCTGTTGCATCTCGATCAGACCCACAGTGTCAAGGCTATTGCA
  ATGTCTCCGTCAGCAGTGTGCACATGAGCCTTCAGAGATACTGTCAGCAGGATTATGTTCTGCACGCCCA
  GGTTTCAGCGTCGTCGTCGCAGCCGTCTGAGGCGGTGGGCCCCGAGTGGTGGCGGCTGGCCGTGCACGTG
  CTGGCTGTGTTCAAACAGCGTGCGTGGCCCGTGCGCCGCGGTGGCCAGGAGGCCTGGGTGCCACGTGCTG
  ACCTAATCTGCGGCTGCCTGCGCCTGCGCCCTGGCGCTGACTACCTCCTGCTGGGCCGTGCTGCGCAGAC
  CCACGACGACGACAACTATGACCCTGCGCGCCTCATCCTGAACCGCCACGGCCTCGCGCTGCCCTGGAGA
  CCACGATGGGCCCGGCCGCTGCGGCGACTGCAGCAGAAGGAGCGCGGAGGTGCCTGCCGAGGCCTGCTGC
  CACCCACACGGAGCCCTGGGCCCAGGAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289693
• Insert Size: 1152 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289693.1, NP_001276622.1
• RefSeq Size: 1761 bp
• RefSeq ORF: 1152 bp
• Locus ID: 243967
• UniProt ID: Q3UQ22
• Cytogenetics: 7 B3
• Gene Summary: Plays a role in neurogenesis. Prevents motor neuron cell body migration out of the neural tube.[UniProtKB/Swiss-Prot Function]