Strada (NM_001252449) Mouse Untagged Clone

CAT#: MC227410

Strada (untagged) - Mouse STE20-related kinase adaptor alpha (Strada), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Strada
• Synonyms: 2610019A05Rik; 6030402H20Rik; AI480680; E130112C09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227410 representing NM_001252449
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTTCTTGCCAATGAGGCGAGCTCAGAGTCCATAGCATCTTTCTCCAAACCAGAGATGATGAGCA
  GCTTTCTTCCAGAGGGCGGGTGTTATGAGCTGCTCACCATCATAGGCAAAGGGTTTGAGGACTTGATGAC
  AGTGAATTTAGCCAGGTACAAACCAACGGGAGAGTATGTGACAGTACGAAGGATCAACCTAGAAGCTTGT
  TCCAATGAGATGGTGACATTCTTGCAGGGGGAGCTTCATGTCTCTAAACTCTTCAGCCATCCCAATATAG
  TACCGTATCGAGCCACCTTCATCGCAGATAATGAGCTGTGGGTCGTCACATCATTTATGGCGTACGGCTC
  TGCAAAGGATCTCATTGGCACACACTTCATGGATGGCATGAATGAACTGGCGATTGCATACATCCTGCAG
  GGGGTGCTAAAGGCCTTAGACTACATCCATCACATGGGCTATGTGCACAGGAGTGTCAAGGCCAGCCACA
  TTCTGATCTCCACGGATGGGAAGGTCTACCTGTCTGGTTTACGCAGCAACCTTAGCATGATTAGTCACGG
  GCAGCGGCAGCGTGCAGTCCACGATTTCCCCAAATATAGTATCAAGGTCCTGCCTTGGCTCAGCCCAGAA
  GTCCTCCAGCAGAATCTTCAGGGTTACGATGCCAAGTCCGATATCTACAGTGTGGGAATCACAGCATGTG
  AACTAGCCAATGGCCATGTCCCCTTCAAAGATATGCCTGCCACTCAGATGCTGCTAGAAAAACTTAATGG
  CACAGTGCCCTGCCTGCTGGACACCAGCACCATCCCTGCCGAGGAGCTGACCATGAGTCCCTCCCGCTCC
  ATCGCCAACCCTGGCCTGAATGACAGCCTGGCTGCTGGTAGCCTCCGGCCTTCCAATGGCGACTCGCCTT
  CCCACCCCTACCACCGAACCTTCTCGCCTCACTTCCACAACTTTGTGGAACAGTGCCTTCAGCGCAACCC
  TGACGCAAGGCCAAACATCAAGCGGCGTGCTTCCGAGGCTTTGCCTGAGTTGCTCCGCCCAGTCACTCCC
  ATCACCAACTTCGAGGGCAGCCAGTCTCAGGATCACAGTGGAATCTTCGGCCTGGTGACAAACCTAGAAG
  ACCTGGAGGTGGATGACTGGGAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252449
• Insert Size: 1149 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252449.1, NP_001239378.1
• RefSeq Size: 2396 bp
• RefSeq ORF: 1149 bp
• Locus ID: 72149
• UniProt ID: Q3UUJ4
• Cytogenetics: 11 E1
• Gene Summary: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two consecutive exons in the 5' coding region and a segment in the 3' coding region, compared to variant 1. The reading frame is not affected and the resulting isoform (3) lacks a segment in the N-terminal region and a segment in the C-terminal region, compared to isoform 1.