Acr (NM_001277245) Mouse Untagged Clone

CAT#: MC227384

Acr (untagged) - Mouse acrosin prepropeptide (Acr), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227384 representing NM_001277245
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCTTACAGATCTTCACGTCCCATAACAGCCGCAGGTACCACGCCTGTGGAGGCAGCCTCCTGA
  ACTCCCACTGGGTGCTCACAGCTGCCCACTGCTTCGATAACAAAAAAAAAGTCTATGACTGGAGACTGGT
  TTTCGGAGCACAAGAAATCGAATATGGAAGAAACAAGCCAGTGAAAGAGCCCCAGCAGGAGAGATATGTG
  CAGAAGATTGTCATCCATGAGAAATACAACGTAGTGACGGAGGGAAATGACATTGCGCTCTTGAAGATCA
  CTCCTCCTGTTACATGTGGGAACTTCATTGGACCCTGCTGTCTACCTCATTTTAAGGCGGGTCCTCCCCA
  AATACCCCACACCTGCTACGTGACTGGGTGGGGATACATAAAAGAGAAGGCCCCCAGGCCATCACCTGTC
  CTGATGGAAGCACGTGTGGACCTCATTGACCTCGACCTGTGTAACTCAACCCAGTGGTACAATGGGCGTG
  TCACATCGACTAATGTGTGTGCAGGGTATCCTGAAGGCAAGATTGACACCTGCCAGGGGGACAGTGGTGG
  GCCTCTCATGTGCAGAGACAACGTCGACAGCCCCTTTGTGGTCGTGGGGATCACGAGCTGGGGGGTAGGC
  TGTGCCCGTGCTAAGCGTCCCGGAGTCTACACAGCCACCTGGGACTACCTGGACTGGATTGCTTCCAAGA
  TCGGCCCTAACGCCTTGCACTTGATTCAGCCAGCCACCCCTCATCCGCCGACTACCCGCCATCCGATGGT
  CTCTTTTCACCCTCCTTCTCTTCGCCCTCCTTGGTATTTCCAACACCTGCCTTCTCGACCGCTTTACCTG
  CGACCACTACGGCCTCTGCTCCATCGGCCGTCTTCGACCCAAACCTCCTCATCACTCATGCCCCTCCTCT
  CGCCCCCAACCCCAGCCCAGCCTGCATCCTTTACCATTGCTACACAACACATGAGGCACCGCACAACGCT
  GTCTTTTGCTCGGCGTCTCCAGCGCCTCATAGAGGCCCTGAAGATGAGAACTTACCCTATGAAACATCCC
  TCCCAGTACAGTGGACCAAGGAACTACCACTACCGCTTCTCCACGTTTGAGCCCCTTTCCAACAAACCCA
  GCGAGCCCTTCCTCCATTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277245
• Insert Size: 1143 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277245.1, NP_001264174.1
• RefSeq Size: 1567 bp
• RefSeq ORF: 1143 bp
• Locus ID: 11434
• Cytogenetics: 15 44.96 cM
• Gene Summary: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' end, which results in the use of a downstream AUG translation initiation site, compared to variant 1. It encodes isoform 3, which is shorter at the N-terminus, compared to isoform 1. Variants 3 and 4 both encode the same isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.